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Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene.
| Title: | Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene. |
| Authors: | Saitoh, Yasushi Browse this author →KAKEN DB | | Miyagi, Satoru Browse this author | | Ariga, Hiroyoshi Browse this author →KAKEN DB | | Tsutsumi, Ken-ichi Browse this author |
| Issue Date: | 1-Dec-2002 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic acids research |
| Volume: | 30 |
| Issue: | 23 |
| Start Page: | 5205 |
| End Page: | 5212 |
| Publisher DOI: | 10.1093/nar/gkf642 |
| PMID: | 12466545 |
| Abstract: | The promoter of the rat aldolase B (AldB) gene functions in vivo as an origin of DNA replication in the cells in which transcription of the gene is repressed. Previously, we identified two closely related DNA-binding proteins, AlF-C1 and AlF-C2, which repressed the AldB gene promoter. We also reported that the binding site of these proteins, site C, is one of the required DNA elements of the AldB gene origin/promoter for autonomously replicating activity in transfected cells. In the present study, we show that AlF-C1 and AlF-C2 bind directly to Orc1, a subunit of the origin recognition complex (ORC). Deletion analyses revealed a functional domain in AlF-C2 for binding to Orc1, which is located separately from the DNA-binding domain. In addition, we found a novel protein-interacting domain in Orc1 required for the binding of AlF-C2, which was conserved in human, mouse and Chinese hamster, but not in Drosophila, frog and yeast. Thus, it is assumed that in mammalian cells, sequence- specific DNA-binding proteins are involved in recruiting ORC to regulate replication initiation and/or transcription repression. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53742 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 有賀 寛芳
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