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DJ-1, a Target Protein for an Endocrine Disrupter, Participates in the Fertilization in Mice
| Title: | DJ-1, a Target Protein for an Endocrine Disrupter, Participates in the Fertilization in Mice |
| Authors: | Okada, Masahiko Browse this author | | Matsumoto, Ken-ichi Browse this author | | Niki, Takeshi Browse this author →KAKEN DB | | Taira, Takahiro Browse this author →KAKEN DB | | Iguchi-Ariga, Sanae M. M. Browse this author →KAKEN DB | | Ariga, Hiroyoshi Browse this author →KAKEN DB |
| Keywords: | DJ-1 | | fertilization | | endocrine disrupter |
| Issue Date: | Jul-2002 |
| Publisher: | The Pharmaceutical Society of Japan |
| Journal Title: | Biological & Pharmaceutical Bulletin |
| Volume: | 25 |
| Issue: | 7 |
| Start Page: | 853 |
| End Page: | 856 |
| Publisher DOI: | 10.1248/bpb.25.853 |
| PMID: | 12132656 |
| Abstract: | DJ-1 was first identified as an activated ras-dependent oncogene product and was later also found to be an infertility-related protein affected by sperm toxicants such as ornidazole (OR) and epichlorohydrin. These findings suggest that DJ-1 has functions in both somatic cells and sperm. In this study, to determine the relationship between DJ-1 and an endocrine disrupter and to determine the functions of DJ-1 in sperm, in vitro fertilization experiments were carried out using eggs and sperm extracted from mice that had or had not been treated with OR. We found that the amount of DJ-1 in sperm and the efficiency of fertilization decreased with the increasing dose of OR to which the mice were exposed. The addition of an anti-mouse DJ-1 serum to sperm solution before the in vitro fertilization reaction with eggs resulted in a decrease in the efficiency of fertilization to about one-third of that when pre-immune serum was added to sperm solution, indicating that DJ-1 participates in the fertilization. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53744 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 有賀 寛芳
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