|
Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Determination of the functional domain of a mouse autonomous replicating sequence.
| Title: | Determination of the functional domain of a mouse autonomous replicating sequence. |
| Authors: | Hayashi, Chiharu Browse this author | | Fujino, Hiromichi Browse this author | | Ogata, Masanori Browse this author | | Sato, Yoshinori Browse this author | | Iguchi-Ariga, Sanae M. M. Browse this author →KAKEN DB | | Ariga, Hiroyoshi Browse this author →KAKEN DB |
| Keywords: | ARS | | c-myc | | replication |
| Issue Date: | Jun-1997 |
| Publisher: | The Pharmaceutical Society of Japan |
| Journal Title: | Biological & pharmaceutical bulletin |
| Volume: | 20 |
| Issue: | 6 |
| Start Page: | 690 |
| End Page: | 693 |
| Publisher DOI: | 10.1248/bpb.20.690 |
| PMID: | 9212992 |
| Abstract: | We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53973 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 有賀 寛芳
|
