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Determination of the functional domain of a mouse autonomous replicating sequence.

84_Ariga_1997_Biol_Pharm_Bull.pdf970.76 kBPDF見る/開く

タイトル: Determination of the functional domain of a mouse autonomous replicating sequence.
著者: Hayashi, Chiharu 著作を一覧する
Fujino, Hiromichi 著作を一覧する
Ogata, Masanori 著作を一覧する
Sato, Yoshinori 著作を一覧する
Iguchi-Ariga, Sanae M. M. 著作を一覧する
Ariga, Hiroyoshi 著作を一覧する
キーワード: ARS
発行日: 1997年 6月
出版者: The Pharmaceutical Society of Japan
誌名: Biological & pharmaceutical bulletin
巻: 20
号: 6
開始ページ: 690
終了ページ: 693
出版社 DOI: 10.1248/bpb.20.690
抄録: We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 有賀 寛芳


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