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Determination of the functional domain of a mouse autonomous replicating sequence.

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Title: Determination of the functional domain of a mouse autonomous replicating sequence.
Authors: Hayashi, Chiharu Browse this author
Fujino, Hiromichi Browse this author
Ogata, Masanori Browse this author
Sato, Yoshinori Browse this author
Iguchi-Ariga, Sanae M. M. Browse this author →KAKEN DB
Ariga, Hiroyoshi Browse this author →KAKEN DB
Keywords: ARS
Issue Date: Jun-1997
Publisher: The Pharmaceutical Society of Japan
Journal Title: Biological & pharmaceutical bulletin
Volume: 20
Issue: 6
Start Page: 690
End Page: 693
Publisher DOI: 10.1248/bpb.20.690
PMID: 09212992
Abstract: We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 有賀 寛芳

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