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Effect of transcriptional regulatory sequences on autonomous replication of plasmids in transient mammalian systems.

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タイトル: Effect of transcriptional regulatory sequences on autonomous replication of plasmids in transient mammalian systems.
著者: Yokoyama, Masahiro 著作を一覧する
Ariga, Hiroyoshi 著作を一覧する
Iguchi-Ariga, Sanae M. M. 著作を一覧する
キーワード: autonomous replication
transcriptional regulatory element
octamer
発行日: 1997年 6月
出版者: The Pharmaceutical Society of Japan
誌名: Biological & pharmaceutical bulletin
巻: 20
号: 6
開始ページ: 613
終了ページ: 620
出版社 DOI: 10.1248/bpb.20.613
抄録: Transcriptional regulatory sequences often influence the efficiency of DNA replication, directly or indirectly, in bacteria, yeast, and animal virus systems. We have tested several transcriptional regulatory sequences for affecting DNA replication, based on pUC vector, in transient systems. Autonomous replication of transfected plasmids was assayed by PCR amplification of the fragments derived from the plasmids, which had replicated in mammalian cells. By this highly efficient method of detecting replicated molecules, pUC19, but not pUC18, showed a week replication activity in transfected cells. Nucleotide sequences around the HindIII site in pUC19 were required for replication. Monomers or dimers of the octamer transcription motif of the mouse immunoglobulin heavy chain gene, inserted in multicloning sites of pUC19, could stimulate replication, while the 4- or 6-mers did not, in contrast to the results on its transcription activity. Other transcriptional elements including AP1, HSE, and E2F also stimulated replication, but neither CRE nor Sp1 binding motif did. These results suggest that at least some of the transcriptional regulatory sequences function as-modulators of DNA replication as well as of transcription.
資料タイプ: article
URI: http://hdl.handle.net/2115/53975
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 有賀 寛芳

 

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