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Effect of transcriptional regulatory sequences on autonomous replication of plasmids in transient mammalian systems.

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Title: Effect of transcriptional regulatory sequences on autonomous replication of plasmids in transient mammalian systems.
Authors: Yokoyama, Masahiro Browse this author
Ariga, Hiroyoshi Browse this author
Iguchi-Ariga, Sanae M. M. Browse this author
Keywords: autonomous replication
transcriptional regulatory element
octamer
Issue Date: Jun-1997
Publisher: The Pharmaceutical Society of Japan
Journal Title: Biological & pharmaceutical bulletin
Volume: 20
Issue: 6
Start Page: 613
End Page: 620
Publisher DOI: 10.1248/bpb.20.613
PMID: 9212977
Abstract: Transcriptional regulatory sequences often influence the efficiency of DNA replication, directly or indirectly, in bacteria, yeast, and animal virus systems. We have tested several transcriptional regulatory sequences for affecting DNA replication, based on pUC vector, in transient systems. Autonomous replication of transfected plasmids was assayed by PCR amplification of the fragments derived from the plasmids, which had replicated in mammalian cells. By this highly efficient method of detecting replicated molecules, pUC19, but not pUC18, showed a week replication activity in transfected cells. Nucleotide sequences around the HindIII site in pUC19 were required for replication. Monomers or dimers of the octamer transcription motif of the mouse immunoglobulin heavy chain gene, inserted in multicloning sites of pUC19, could stimulate replication, while the 4- or 6-mers did not, in contrast to the results on its transcription activity. Other transcriptional elements including AP1, HSE, and E2F also stimulated replication, but neither CRE nor Sp1 binding motif did. These results suggest that at least some of the transcriptional regulatory sequences function as-modulators of DNA replication as well as of transcription.
Type: article
URI: http://hdl.handle.net/2115/53975
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 有賀 寛芳

 

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