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Expression of Foreign Genes in Lamprey Embryos: An Approach to Study Evolutionary Changes in Gene Regulation

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Title: Expression of Foreign Genes in Lamprey Embryos: An Approach to Study Evolutionary Changes in Gene Regulation
Authors: Kusakabe, Rie Browse this author
Tochinai, Shin2 Browse this author →KAKEN DB
Kuratani, Shigeru Browse this author
Authors(alt): 栃内, 新2
Issue Date: 20-Mar-2003
Publisher: Wiley-Liss, Inc.
Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume: 296B
Issue: 1
Start Page: 87
End Page: 97
Publisher DOI: 10.1002/jez.b.11
Abstract: Evolution in development can be viewed as a sequence of changes in gene regulation. To investigate the cross-species compatibility of 5’ upstream regulatory regions, we introduced exogenous gene constructs derived from a gnathostome genome into fertilized eggs of the Japanese lamprey, Lampetra japonica, a sister group of the gnathostomes. Eggs were injected with gene constructs in which a sequence encoding the green fluorescent protein (GFP) had been located downstream of either a virus promoter or 5’ regulatory regions of medaka actin genes. Reporter gene expression was recorded for more than a month starting two days after injection. Although the expression patterns were highly mosaic and differed among individuals, GFP was expressed predominantly in the striated muscles of lamprey embryos when driven by the 5’ upstream regions of the medaka muscle actin genes. This implies that a pan-vertebrate muscle-specific gene regulatory mechanism may have evolved before the agnathan/gnathostome divergence. This gene-transfer technique potentially facilitates the visualization of cells in various differentiating tissues throughout development. The introduction of developmental genes of the lamprey or other animals into lamprey embryos is another potentially important application, one that could provide us with information on the evolutionary changes in functions of genes or gene cascades. J. Exp. Zool. (Mol.Dev. Evol.) 296B:87–97, 2003
Rights: Copyright (c) 2003 Wiley-Liss, Inc.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 栃内 新

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