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c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/54049

Title: c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
Authors: Iguchi-Ariga, Sanae M. M. Browse this author →KAKEN DB
Itani, Teru Browse this author
Yamaguchi, Masamitsu Browse this author
Ariga, Hiroyoshi Browse this author →KAKEN DB
Issue Date: 25-Jun-1987
Publisher: Oxford University Press
Journal Title: Nucleic acids research
Volume: 15
Issue: 12
Start Page: 4889
End Page: 4899
Publisher DOI: 10.1093/nar/15.12.4889
PMID: 03037484
Abstract: Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
Type: article
URI: http://hdl.handle.net/2115/54049
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 有賀 寛芳

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