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Adenovirus DNA synthesized in the presence of aphidicolin.

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タイトル: Adenovirus DNA synthesized in the presence of aphidicolin.
著者: Oguro, Mieko 著作を一覧する
Yamashita, Tadashi 著作を一覧する
Ariga, Hiroyoshi 著作を一覧する
Nagano, Hiroshi 著作を一覧する
発行日: 1984年 1月25日
出版者: Oxford University Press
誌名: Nucleic acids research
巻: 12
号: 2
開始ページ: 1077
終了ページ: 1086
出版社 DOI: 10.1093/nar/12.2.1077
抄録: Adenovirus types 2 and 5 DNA synthesized in vivo and in vitro in the presence of aphidicolin were studied. Inhibition of adenoviral DNA synthesis by aphidicolin was only 70% even at a concentration of 30 micrograms/ml of aphidicolin, at which the cellular DNA synthesis was completely inhibited. When initiation of the viral DNA synthesis was synchronized with hydroxyurea and labeled with [3H]thymidine for 60 min, the viral DNA synthesized in the presence of 30 micrograms/ml of aphidicolin was not of full length (35 kb) but small (approximately 12 kb) by analysis of alkaline sucrose density gradient centrifugation. When initiation of the viral DNA synthesis was not synchronized, the viral DNAs ranging from full size to 12 kb were synthesized in the presence of aphidicolin, indicating that the nascent DNAs longer than about 12 kb can continue to elongate in the presence of aphidicolin. This 12 kb DNA was not derived from the degradation products of newly synthesized full size adenoviral DNA. The viral DNA synthesis was restored and the full size of adenoviral DNA was attained within 15 min following removal of aphidicolin. About 20% of the entire viral genome length from the 5'-end was not inhibited by aphidicolin, while the synthesis of interior fragments of the adenoviral DNA was markedly inhibited by aphidicolin, judging from the electrophoretic pattern on neutral agarose gel after digestion of DNA with Hind III. These results indicate that aphidicolin inhibits adenoviral DNA replication at the internal region located approximately 20-30% from both terminals.
Relation (URI): http://nar.oxfordjournals.org/content/12/2/1077.abstract
資料タイプ: article
URI: http://hdl.handle.net/2115/54052
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 有賀 寛芳

 

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