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Unperverted synthesis of complex sphingolipids is essential for cell survival under nitrogen starvation

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Title: Unperverted synthesis of complex sphingolipids is essential for cell survival under nitrogen starvation
Authors: Yamagata, Maki Browse this author
Obara, Keisuke Browse this author →KAKEN DB
Kihara, Akio Browse this author →KAKEN DB
Issue Date: Aug-2013
Publisher: Wiley-Blackwell
Journal Title: Genes to Cells
Volume: 18
Issue: 8
Start Page: 650
End Page: 659
Publisher DOI: 10.1111/gtc.12062
PMID: 23692275
Abstract: Changes in membrane dynamics are known to occur in cells faced with starvation. However, the functions of the major lipid components of biological membranes, sphingolipids, during the starvation response remain unclear. In this study, we found that yeast cells lacking genes encoding mannosylinositol phosphorylceramide (MIPC) synthases (csg1 csh1) underwent rapid cell death upon nitrogen starvation, but not upon carbon starvation or carbon and nitrogen starvation. Addition of NaN3 prevented the nitrogen starvation-induced cell death of the csg1 csh1 cells, indicating that energy production is required for this rapid cell death. IPC) species containing phytosphingosine. Removing Ca2+ by treating the cells with a calcium chelator or by changing the medium to a Ca2+-free medium before nitrogen starvation rescued the cells from death. Approximately half of the cells died shortly after collapse of the vacuole, whereas in the other half, morphological changes in the cytoplasm preceded vacuole disruption. Because the vacuole is the major Ca2+ storage organelle, we suggest that the vacuole is involved in the cell death either directly or indirectly. We report here that normal synthesis of complex sphingolipids is important for cell survival in nitrogen-starved medium.
Rights: The definitive version is available at
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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