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Transcriptional regulation of the legumain gene by p53 in HCT116 cells

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Title: Transcriptional regulation of the legumain gene by p53 in HCT116 cells
Authors: Yamane, Takuya Browse this author
Murao, Sato Browse this author
Kato-Ose, Izumi Browse this author
Kashima, Lisa Browse this author →KAKEN DB
Yuguchi, Motoki Browse this author
Kozuka, Miyuki Browse this author
Takeuchi, Keisuke Browse this author →KAKEN DB
Ogita, Hisakazu Browse this author →KAKEN DB
Ohkubo, Iwao Browse this author →KAKEN DB
Ariga, Hiroyoshi Browse this author →KAKEN DB
Keywords: Legumain
p53
Doxorubicin
Issue Date: 6-Sep-2013
Publisher: Academic press inc elsevier science
Journal Title: Biochemical and biophysical research communications
Volume: 438
Issue: 4
Start Page: 613
End Page: 618
Publisher DOI: 10.1016/j.bbrc.2013.08.007
PMID: 23942113
Abstract: Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was found to be highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro and in vivo. In this study, we found a p53-binding site in intron 1 of the human legumain gene using computational analysis. To determine whether transcription of the legumain gene is regulated by p53, HCT116 cells were transfected with p53 siRNA and the effect of knockdown of p53 expression on legumain expression was examined. The results showed that expression levels of both legumain mRNA and protein were decreased in the siRNA-treated cells. Furthermore, enzyme activity of legumain was also increased by doxorubicin and its activity was reduced by knockdown of p53 in HCT116 cells. These results suggest that legumain expression and its enzyme activity are regulated by p53. (C) 2013 Elsevier Inc. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/54547
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山根 拓也

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