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Three Testis-Specific Paralogous Serine Proteases Play Different Roles in Murine Spermatogenesis and Are Involved in Germ Cell Survival During Meiosis
Title: | Three Testis-Specific Paralogous Serine Proteases Play Different Roles in Murine Spermatogenesis and Are Involved in Germ Cell Survival During Meiosis |
Authors: | Yoneda, Ryoma Browse this author | Takahashi, Takayuki Browse this author →KAKEN DB | Matsui, Hitoshi Browse this author | Takano, Naoharu Browse this author | Hasebe, Yuko Browse this author | Ogiwara, Katsueki Browse this author →KAKEN DB | Kimura, Atsushi P. Browse this author →KAKEN DB |
Keywords: | apoptosis | germ cell | localization | meiosis | organ culture | protease | Prss | spermatogenesis | Tessp |
Issue Date: | May-2013 |
Publisher: | The Society for the Study of Reproduction |
Journal Title: | Biology of Reproduction |
Volume: | 88 |
Issue: | 5 |
Start Page: | 118 |
End Page: | 118 |
Publisher DOI: | 10.1095/biolreprod.112.106328 |
PMID: | 23536369 |
Abstract: | Spermatogenesis is a complex process that generates spermatozoa; its molecular mechanisms are not completely understood. Here we focused on the functions of three testis-specific serine proteases: Prss42/Tessp-2, Prss43/Tessp-3, and Prss44/Tessp-4. These protease genes, which constitute a gene cluster on chromosome 9F2-F3, were presumed to be paralogs and were expressed only in the testis. By investigating their mRNA distribution, we found that all three genes were expressed in primary and secondary spermatocytes. However, interestingly, the translated proteins were produced at different locations. Prss42/Tessp-2 was found in the membranes and cytoplasm of secondary spermatocytes and spermatids, whereas Prss43/Tessp-3 was present only in the membranes of spermatocytes and spermatids. Prss44/Tessp-4 was detected in the cytoplasm of spermatocytes and spermatids. To assess the roles of these proteases in spermatogenesis, we used organ culture of mouse testis fragments. Adding antibodies against Prss42/Tessp-2 and Prss43/Tessp-3 resulted in meiotic arrest at the stage when each protease was beginning to be translated. Furthermore, the number of apoptotic cells dramatically increased after the addition of these antibodies. These results strongly suggest that the three paralogous Prss/Tessp proteases play different roles in spermatogenesis and that Prss42/Tessp-2 and Prss43/Tessp-3 are required for germ cell survival during meiosis. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/54627 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 敦
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