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Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins
Title: | Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins |
Authors: | Ohno, Kaoru Browse this author →KAKEN DB | Sawada, Jun-ichi Browse this author | Takiya, Shigeharu Browse this author →KAKEN DB | Kimoto, Mai Browse this author | Matsumoto, Akiko Browse this author | Tsubota, Takuya Browse this author | Uchino, Keiro Browse this author | Hui, Chi-chung Browse this author | Sezutsu, Hideki Browse this author →KAKEN DB | Handa, Hiroshi Browse this author →KAKEN DB | Suzuki, Yoshiaki Browse this author →KAKEN DB |
Keywords: | Gene transcription | Homeobox | Silkworm | Tissue-specific transcription factors | Transcription enhancers | Fibroin Gene | Homeodomain | Silk gland factor-2 (SGF-2) |
Issue Date: | 1-Nov-2013 |
Publisher: | Amer soc biochemistry molecular biology inc |
Journal Title: | Journal of biological chemistry |
Volume: | 288 |
Issue: | 44 |
Start Page: | 31581 |
End Page: | 31591 |
Publisher DOI: | 10.1074/jbc.M113.514471 |
Abstract: | SGF-2 binds to promoter elements governing posterior silk gland-specific expression of the fibroin gene in Bombyx mori. Wepurified SGF-2 and showed that SGF-2 contains at least four gene products: the silkworm orthologues of LIM homeodomain protein Awh, LIM domain-binding protein (Ldb), a sequence-specific single-stranded DNA-binding protein (Lcaf), and the silk protein P25/fibrohexamerin (fhx). Using co-expression of these factors in Sf9 cells, Awh, Ldb, and Lcaf proteins were co-purified as a ternary complex that bound to the enhancer sequence in vitro. Lcaf interacts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation in yeast. Misexpression of Awh in transgenic silkworms induces ectopic expression of the fibroin gene in the middle silk glands, where Ldb and Lcaf are expressed. Taken together, this study demonstrates that SGF-2 is a multisubunit activator complex containing Awh. Moreover, our results suggest that the Ldb.Lcaf protein complex serves as a scaffold to facilitate communication between transcriptional control elements. |
Rights: | This research was originally published in Journal of Biological Chemistry. Shigeharu Takiya(s). Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins. Journal of Biological Chemistry. Year;2013 Vol:288(44)pp31518-pp31519. Copylight ©2013 the American Society for Biochemistry and Molecular Biology. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/54948 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 瀧谷 重治
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