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Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins

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Title: Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins
Authors: Ohno, Kaoru Browse this author →KAKEN DB
Sawada, Jun-ichi Browse this author
Takiya, Shigeharu Browse this author →KAKEN DB
Kimoto, Mai Browse this author
Matsumoto, Akiko Browse this author
Tsubota, Takuya Browse this author
Uchino, Keiro Browse this author
Hui, Chi-chung Browse this author
Sezutsu, Hideki Browse this author →KAKEN DB
Handa, Hiroshi Browse this author →KAKEN DB
Suzuki, Yoshiaki Browse this author →KAKEN DB
Keywords: Gene transcription
Homeobox
Silkworm
Tissue-specific transcription factors
Transcription enhancers
Fibroin Gene
Homeodomain
Silk gland factor-2 (SGF-2)
Issue Date: 1-Nov-2013
Publisher: Amer soc biochemistry molecular biology inc
Journal Title: Journal of biological chemistry
Volume: 288
Issue: 44
Start Page: 31581
End Page: 31591
Publisher DOI: 10.1074/jbc.M113.514471
Abstract: SGF-2 binds to promoter elements governing posterior silk gland-specific expression of the fibroin gene in Bombyx mori. Wepurified SGF-2 and showed that SGF-2 contains at least four gene products: the silkworm orthologues of LIM homeodomain protein Awh, LIM domain-binding protein (Ldb), a sequence-specific single-stranded DNA-binding protein (Lcaf), and the silk protein P25/fibrohexamerin (fhx). Using co-expression of these factors in Sf9 cells, Awh, Ldb, and Lcaf proteins were co-purified as a ternary complex that bound to the enhancer sequence in vitro. Lcaf interacts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation in yeast. Misexpression of Awh in transgenic silkworms induces ectopic expression of the fibroin gene in the middle silk glands, where Ldb and Lcaf are expressed. Taken together, this study demonstrates that SGF-2 is a multisubunit activator complex containing Awh. Moreover, our results suggest that the Ldb.Lcaf protein complex serves as a scaffold to facilitate communication between transcriptional control elements.
Rights: This research was originally published in Journal of Biological Chemistry. Shigeharu Takiya(s). Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins. Journal of Biological Chemistry. Year;2013 Vol:288(44)pp31518-pp31519. Copylight ©2013 the American Society for Biochemistry and Molecular Biology.
Type: article (author version)
URI: http://hdl.handle.net/2115/54948
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 瀧谷 重治

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