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AHCYL2 (long-IRBIT) as a potential regulator of the electrogenic Na+-HCO3- cotransporter NBCe1-B
| Title: | AHCYL2 (long-IRBIT) as a potential regulator of the electrogenic Na+-HCO3- cotransporter NBCe1-B |
| Authors: | Yamaguchi, Soichiro Browse this author | | Ishikawa, Toru Browse this author →KAKEN DB |
| Keywords: | NBCe1-B | | Long-IRBIT | | IRBIT | | Intracellular Mg2+ | | Bovine parotid acinar cell | | Whole-cell patch-clamp |
| Issue Date: | 3-Mar-2014 |
| Publisher: | Elsevier |
| Journal Title: | Febs letters |
| Volume: | 588 |
| Issue: | 5 |
| Start Page: | 672 |
| End Page: | 677 |
| Publisher DOI: | 10.1016/j.febslet.2013.12.036 |
| PMID: | 24472682 |
| Abstract: | Although AHCYL2 (long-IRBIT) is highly homologous to IRBIT, which regulates ion-transporting proteins including the electrogenic Na+-HCO(3)(-)cotransporter NBCe1-B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1-B in bovine parotid acinar cells using yeast two-hybrid, immunofluorescence confocal microscopy and co-immunoprecipitation analyses. Whole-cell patch-clamp experiments revealed that co-expression of AHCYL2 reduces the apparent affinity for intracellular Mg2+ in inhibition of NBCe1-B currents specifically in a HCO3- -deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1-B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/55272 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山口 聡一郎
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