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A laminaribiose-hydrolyzing enzyme, AkLab, from the common sea hare Aplysia kurodai and its transglycosylation activity

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Title: A laminaribiose-hydrolyzing enzyme, AkLab, from the common sea hare Aplysia kurodai and its transglycosylation activity
Authors: Kumagai, Yuya Browse this author
Satoh, Takuya Browse this author
Inoue, Akira Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Keywords: Gastropod
Sea hare
Aplysia
Laminaribiose
beta-Glucosidase
Issue Date: Jan-2014
Publisher: Elsevier science inc
Journal Title: Comparative biochemistry and physiology b-biochemistry & molecular biology
Volume: 167
Start Page: 1
End Page: 7
Publisher DOI: 10.1016/j.cbpb.2013.07.008
PMID: 23912026
Abstract: Endo-beta-1,3-glucanases (laminarinase, EC 3.2.1.6) from marine molluscs specifically degrades laminarin from brown algae producing laminaribiose and glucose, but hardly degrades laminaribiose. For the complete depolymerization of laminarin, other enzymes that can hydrolyze laminaribiose appear to be necessary. In the present study, we successfully isolated a laminaribiose-hydrolyzing enzyme from the digestive fluid of a marine gastropod Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatographies. This enzyme, AkLab, named after the scientific name of this animal and substrate specificity toward laminaribiose, shows an approximate molecular mass of 110 kDa on SDS-PAGE, and optimum pH and temperature at around pH 5.5 and 50 degrees C, respectively. AkLab rapidly hydrolyzes laminaribiose and p-nitrophenyl-beta-D-glucoside, and slowly cellobiose, gentiobiose and lactose, but not sucrose and maltose. AkLab shows high transglycosylation activity and can produce a series of laminarioligosaccharides larger than laminaritetraose from laminaribiose (a donor substrate) and laminaritriose (an acceptor substrate). This enzyme is suggested to be a member of glycosyl hydrolase family 1 by the analysis of partial amino-acid sequences. (C) 2013 Elsevier Inc. All rights reserved.
Relation: http://www.elsevier.com/locate/cbpb
Type: article (author version)
URI: http://hdl.handle.net/2115/55292
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 尾島 孝男

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