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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
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The potential of glycerol in freezing preservation of turbine oil-degrading bacterial consortium and the ability of the revised consortium to degrade petroleum wastes
| Title: | The potential of glycerol in freezing preservation of turbine oil-degrading bacterial consortium and the ability of the revised consortium to degrade petroleum wastes |
| Authors: | Kurachi, Kumiko Browse this author | | Hosokawa, Reia Browse this author | | Takahashi, Marina Browse this author | | Okuyama, Hidetoshi Browse this author →KAKEN DB |
| Keywords: | Bacterial consortia | | Biodegradation | | Crude oil | | Culture revival | | Glycerol stock | | Turbine oil | | Wastewater |
| Issue Date: | Mar-2014 |
| Publisher: | Elsevier, B.V. |
| Journal Title: | International Biodeterioration & Biodegradation |
| Volume: | 88 |
| Start Page: | 77 |
| End Page: | 82 |
| Publisher DOI: | 10.1016/j.ibiod.2013.12.005 |
| Abstract: | The turbine oil (TuO)-degrading bacterial consortium Tank-2 (original Tank-2) was preserved as a glycerol stock at -80 degrees C from 2009 to 2012. Storage methods have been unavailable so far for any TuO-degrading bacterial consortia or isolates. To evaluate the usefulness of glycerol stock, the original Tank-2 consortium frozen in glycerol at -80 degrees C was thawed and then revived by repeated culture in mineral salts medium (MSM) containing 0.5% (w/w) TuO (revived Tank-2). The revived Tank-2 consortium exhibited a high activity to degrade TuO, which was equivalent to that of original Tank-2. It also degraded car engine oil, used car engine oil, Arabian light and Vityaz crude oils and TuO in wastewater. These results indicated that a glycerol stock at -80 degrees C was useful for storing Tank-2. PCR-denaturing gradient gel electrophoresis (DGGE) that targeted the V3 regions of 16S rRNA gene sequences showed that the DGGE band profiles of principal bacteria were significantly different between the original and revived Tank-2 consortia and between the revived Tank-2 culture grown in MSM containing TuO and that grown in MSM containing other types of petroleum products. This suggested that bacterial strains inherently residing in Tank-2 could adjust their compositions based on the storage and culture conditions. (C) 2013 Elsevier Ltd. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/55385 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 奥山 英登志
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