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Regiospecificities and Prenylation Mode Specificities of the Fungal Indole Diterpene Prenyltransferases AtmD and PaxD

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タイトル: Regiospecificities and Prenylation Mode Specificities of the Fungal Indole Diterpene Prenyltransferases AtmD and PaxD
著者: Liu, Chengwei 著作を一覧する
Minami, Atsushi 著作を一覧する
Noike, Motoyoshi 著作を一覧する
Toshima, Hiroaki 著作を一覧する
Oikawa, Hideaki 著作を一覧する
Dairi, Tohru 著作を一覧する
発行日: 2013年12月
出版者: Amer soc microbiology
誌名: Applied and environmental microbiology
巻: 79
号: 23
開始ページ: 7298
終了ページ: 7304
出版社 DOI: 10.1128/AEM.02496-13
抄録: We recently reported the function of paxD, which is involved in the paxilline (compound 1) biosynthetic gene cluster in Penicillium paxilli. Recombinant PaxD catalyzed a stepwise regular-type diprenylation at the 21 and 22 positions of compound 1 with dimethylallyl diphosphate (DMAPP) as the prenyl donor. In this study, atmD, which is located in the aflatrem (compound 2) biosynthetic gene cluster in Aspergillus flavus and encodes an enzyme with 32% amino acid identity to PaxD, was characterized using recombinant enzyme. When compound 1 and DMAPP were used as substrates, two major products and a trace of minor product were formed. The structures of the two major products were determined to be reversely monoprenylated compound 1 at either the 20 or 21 position. Because compound 2 and beta-aflatrem (compound 3), both of which are compound 1-related compounds produced by A. flavus, have the same prenyl moiety at the 20 and 21 position, respectively, AtmD should catalyze the prenylation in compound 2 and 3 biosynthesis. More importantly and surprisingly, AtmD accepted paspaline (compound 4), which is an intermediate of compound 1 biosynthesis that has a structure similar to that of compound 1, and catalyzed a regular monoprenylation of compound 4 at either the 21 or 22 position, though the reverse prenylation was observed with compound 1. This suggests that fungal indole diterpene prenyltransferases have the potential to alter their position and regular/ reverse specificities for prenylation and could be applicable for the synthesis of industrially useful compounds.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/56461
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大利 徹

 

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