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Lorenzo's oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid
| Title: | Lorenzo's oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid |
| Authors: | Sassa, Takayuki Browse this author →KAKEN DB | | Wakashima, Takeshi Browse this author | | Ohno, Yusuke Browse this author | | Kihara, Akio Browse this author →KAKEN DB |
| Keywords: | ABCD1 | | erucic acid | | oleic acid | | peroxisome | | sphingolipids | | X-linked adrenoleukodystrophy | | elongation of very long-chain fatty acid 1 |
| Issue Date: | Mar-2014 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | Journal of Lipid Research |
| Volume: | 55 |
| Issue: | 3 |
| Start Page: | 524 |
| End Page: | 530 |
| Publisher DOI: | 10.1194/jlr.M044586 |
| Abstract: | X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal disorder caused by impaired degradation of very long-chain fatty acids (VLCFAs) due to mutations in the ABCD1 gene responsible for VLCFA transport into peroxisomes. Lorenzo's oil, a 4: 1 mixture of glyceryl trioleate and glyceryl trierucate, has been used to reduce the saturated VLCFA level in the plasma of X-ALD patients; however, the mechanism by which this occurs remains elusive. We report the biochemical characterization of Lorenzo's oil activity toward elongation of very long-chain fatty acid (ELOVL) 1, the primary enzyme responsible for the synthesis of saturated and monounsaturated VLCFAs. Oleic and erucic acids inhibited ELOVL1, and, moreover, their 4:1 mixture (the FA composition of Lorenzo's oil) exhibited the most potent inhibitory activity. The kinetics analysis revealed that this was a mixed (not a competitive) inhibition. At the cellular level, treatment with the 4:1 mixture reduced the level of SM with a saturated VLCFA accompanied by an increased level of SM with a monounsaturated VLCFA, probably due to the incorporation of erucic acid into the FA elongation cycle.(jlr) These results suggest that inhibition of ELOVL1 may be an underlying mechanism by which Lorenzo's oil exerts its action. |
| Rights: | This research was originally published in Journal of Lipid Research. Sassa, T., et al. Lorenzo's oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid. Journal of Lipid Research. 2014; 55(3):524-530. (c) 2014 the American Society for Biochemistry and Molecular Biology. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/56592 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木原 章雄
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