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Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain
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| Title: | Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain |
| Authors: | Tamaru, Mina Browse this author | | Akita, Hidetaka Browse this author →KAKEN DB | | Nakatani, Taichi Browse this author | | Kajimoto, Kazuaki Browse this author →KAKEN DB | | Sato, Yusuke Browse this author | | Hatakeyama, Hiroto Browse this author →KAKEN DB | | Harashima, Hideyoshi Browse this author →KAKEN DB |
| Keywords: | brain | | DNA | | oligonucleotide | | delivery | | nanoparticles |
| Issue Date: | 8-Sep-2014 |
| Publisher: | Dove Medical Press |
| Journal Title: | International Journal of Nanomedicine |
| Volume: | 9 |
| Start Page: | 4267 |
| End Page: | 4276 |
| Publisher DOI: | 10.2147/IJN.S65402 |
| Abstract: | An innovative drug delivery technology is urgently needed to satisfy unmet medical needs in treating various brain disorders. As a fundamental carrier for plasmid DNA or nucleic acids, we developed a liposomal nanoparticle (multifunctional envelope-type nano device [MEND]) containing a proton-ionizable amino lipid (YSK-MEND). Here we report on the impact of apolipoprotein E (ApoE) modification on the function of YSK-MEND in terms of targeting brain cells. The cellular uptake and function of YSK-MEND encapsulating short interference RNA or plasmid DNA were significantly improved as a result of ApoE modification in mouse neuron-derived cell lines (Neuro-2a and CAD). Intracerebroventricular administration of ApoE-modified YSK-MEND (ApoE/YSK-MEND) encapsulating plasmid DNA also resulted in higher transgene expression in comparison with YSK-MEND that was not modified with ApoE. Moreover, observation of fluorescence-labeled ApoE/YSK-MEND and expression of mCherry (fluorescence protein) derived from plasmid DNA indicated that this carrier might be useful for delivering and conferring transgene expression in neural stem cells and/or neural progenitor cells. Thus, this system may be a useful tool for the treatment of neurodegenerative disease. |
| Rights: | http://creativecommons.org/licenses/by-nc/3.0/us/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/57260 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 秋田 英万
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