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Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/57260

Title: Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain
Authors: Tamaru, Mina Browse this author
Akita, Hidetaka Browse this author →KAKEN DB
Nakatani, Taichi Browse this author
Kajimoto, Kazuaki Browse this author →KAKEN DB
Sato, Yusuke Browse this author
Hatakeyama, Hiroto Browse this author →KAKEN DB
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: brain
DNA
oligonucleotide
delivery
nanoparticles
Issue Date: 8-Sep-2014
Publisher: Dove Medical Press
Journal Title: International Journal of Nanomedicine
Volume: 9
Start Page: 4267
End Page: 4276
Publisher DOI: 10.2147/IJN.S65402
Abstract: An innovative drug delivery technology is urgently needed to satisfy unmet medical needs in treating various brain disorders. As a fundamental carrier for plasmid DNA or nucleic acids, we developed a liposomal nanoparticle (multifunctional envelope-type nano device [MEND]) containing a proton-ionizable amino lipid (YSK-MEND). Here we report on the impact of apolipoprotein E (ApoE) modification on the function of YSK-MEND in terms of targeting brain cells. The cellular uptake and function of YSK-MEND encapsulating short interference RNA or plasmid DNA were significantly improved as a result of ApoE modification in mouse neuron-derived cell lines (Neuro-2a and CAD). Intracerebroventricular administration of ApoE-modified YSK-MEND (ApoE/YSK-MEND) encapsulating plasmid DNA also resulted in higher transgene expression in comparison with YSK-MEND that was not modified with ApoE. Moreover, observation of fluorescence-labeled ApoE/YSK-MEND and expression of mCherry (fluorescence protein) derived from plasmid DNA indicated that this carrier might be useful for delivering and conferring transgene expression in neural stem cells and/or neural progenitor cells. Thus, this system may be a useful tool for the treatment of neurodegenerative disease.
Rights: http://creativecommons.org/licenses/by-nc/3.0/us/
Type: article
URI: http://hdl.handle.net/2115/57260
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 秋田 英万

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