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Localization of exogenous DNA to mitochondria in skeletal muscle following hydrodynamic limb vein injection

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Title: Localization of exogenous DNA to mitochondria in skeletal muscle following hydrodynamic limb vein injection
Authors: Yasuzaki, Yukari Browse this author
Yamada, Yuma Browse this author →KAKEN DB
Kanefuji, Tsutomu Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Mitochondria disease
Mitochondrial gene delivery
Hydrodynamic limb vein injection
Mitochondrial gene therapy
Issue Date: 28-Dec-2013
Publisher: Elsevier science bv
Journal Title: Journal of controlled release
Volume: 172
Issue: 3
Start Page: 805
End Page: 811
Publisher DOI: 10.1016/j.jconrel.2013.09.029
PMID: 24100263
Abstract: Mitochondrial genetic disorders are a major cause of mitochondrial diseases. It is therefore likely that mitochondrial gene therapy will be useful for the treatment of such diseases. Here, we report on the possibility of mitochondrial gene delivery in skeletal muscle using hydrodynamic limb vein (HLV) injection. The HLV injection procedure, a useful method for transgene expression in skeletal muscle, involves the rapid injection of a large volume of naked plasmid DNA (pDNA) into the distal vein of a limb. We hypothesized that the technique could be used to deliver pDNA not only to nuclei but also to mitochondria, since cytosolic pDNA that is internalized by the method may be able to overcome mitochondrial membrane. We determined if pDNA could be delivered to myofibrillar mitochondria by HLV injection by PCR analysis. Mitochondrial toxicity assays showed that the HLV injection had no influence on mitochondrial function. These findings indicate that HLV injection promises to be a useful technique for in vivo mitochondrial gene delivery. (C) 2013 Elsevier B.V. All rights reserved.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 勇磨

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