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A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line
| Title: | A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line |
| Authors: | Yamada, Yuma Browse this author →KAKEN DB | | Tabata, Mai Browse this author | | Yasuzaki, Yukari Browse this author | | Nomura, Masatoshi Browse this author →KAKEN DB | | Shibata, Atsushi Browse this author | | Ibayashi, Yuta Browse this author | | Taniguchi, Yosuke Browse this author →KAKEN DB | | Sasaki, Shigeki Browse this author →KAKEN DB | | Harashima, Hideyoshi Browse this author →KAKEN DB |
| Keywords: | MIN6 cell | | Nucleic acids delivery | | Multifunctional envelope-type nano device (MEND) | | Pancreatic beta cells | | Diabetes | | microRNA |
| Issue Date: | Aug-2014 |
| Publisher: | Elsevier |
| Journal Title: | Biomaterials |
| Volume: | 35 |
| Issue: | 24 |
| Start Page: | 6430 |
| End Page: | 6438 |
| Publisher DOI: | 10.1016/j.biomaterials.2014.04.017 |
| PMID: | 24816283 |
| Abstract: | Pancreatic beta cells secrete insulin in response to glucose levels and thus are involved in controlling blood glucose levels. A line of pancreatic beta cells "MIN6" has been used in studies related to the function of beta cells and diabetes therapy. Regulating gene expression in MIN6 cells could accelerate these studies, but an efficient method for the transfection of nucleic acids targeted to MINE cells is required. We report here on a liposome-based carrier targeted to pancreatic beta cells (Multifunctional envelope-type nano device for pancreatic beta cells, beta-MEND). We identified a lipid composition for use in preparing the beta-MEND, which permits the particles to be efficiently internalized into MIN6, as evidenced by flow cytometry analyses. Intracellular observation by confocal laser scanning microscopy showed that the beta-MEND efficiently delivered the oligo nucleic acids to the cytosol of MINE cells. Moreover, using a beta-MEND encapsulating a 2'-O-Methyl RNA complementary to a microRNA that suppresses insulin secretion, the knockdown of the targeted microRNA and an up-regulation of insulin secretion were observed in MINE. Thus, the beta-MEND holds promise as an efficient system for delivering nucleic acids targeted to MIN6 and can contribute to research and therapy aimed at diabetes. (C) 2014 Elsevier Ltd. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57456 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 勇磨
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