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A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

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Title: A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line
Authors: Yamada, Yuma Browse this author →KAKEN DB
Tabata, Mai Browse this author
Yasuzaki, Yukari Browse this author
Nomura, Masatoshi Browse this author →KAKEN DB
Shibata, Atsushi Browse this author
Ibayashi, Yuta Browse this author
Taniguchi, Yosuke Browse this author →KAKEN DB
Sasaki, Shigeki Browse this author →KAKEN DB
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: MIN6 cell
Nucleic acids delivery
Multifunctional envelope-type nano device (MEND)
Pancreatic beta cells
Issue Date: Aug-2014
Publisher: Elsevier
Journal Title: Biomaterials
Volume: 35
Issue: 24
Start Page: 6430
End Page: 6438
Publisher DOI: 10.1016/j.biomaterials.2014.04.017
PMID: 24816283
Abstract: Pancreatic beta cells secrete insulin in response to glucose levels and thus are involved in controlling blood glucose levels. A line of pancreatic beta cells "MIN6" has been used in studies related to the function of beta cells and diabetes therapy. Regulating gene expression in MIN6 cells could accelerate these studies, but an efficient method for the transfection of nucleic acids targeted to MINE cells is required. We report here on a liposome-based carrier targeted to pancreatic beta cells (Multifunctional envelope-type nano device for pancreatic beta cells, beta-MEND). We identified a lipid composition for use in preparing the beta-MEND, which permits the particles to be efficiently internalized into MIN6, as evidenced by flow cytometry analyses. Intracellular observation by confocal laser scanning microscopy showed that the beta-MEND efficiently delivered the oligo nucleic acids to the cytosol of MINE cells. Moreover, using a beta-MEND encapsulating a 2'-O-Methyl RNA complementary to a microRNA that suppresses insulin secretion, the knockdown of the targeted microRNA and an up-regulation of insulin secretion were observed in MINE. Thus, the beta-MEND holds promise as an efficient system for delivering nucleic acids targeted to MIN6 and can contribute to research and therapy aimed at diabetes. (C) 2014 Elsevier Ltd. All rights reserved.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 勇磨

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