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Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake

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Title: Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake
Authors: Kubo, Kyoko Browse this author →KAKEN DB
Kojima, Hisaya Browse this author →KAKEN DB
Fukui, Manabu Browse this author →KAKEN DB
Keywords: Meromictic lake
Sulfate-reducing bacteria
Issue Date: Oct-2014
Publisher: Elsevier
Journal Title: Systematic and Applied Microbiology
Volume: 37
Issue: 7
Start Page: 510
End Page: 519
Publisher DOI: 10.1016/j.syapm.2014.05.008
PMID: 25034383
Abstract: The vertical distribution of sulfate-reducing bacteria was investigated in a shallow, eutrophic, meromictic lake, Lake Harutori, located in a residential area of Kushiro, Japan. A steep chemocline, characterized by gradients of oxygen, sulfide and salinity, was found at a depth of 3.5-4.0 m. The sulfide concentration at the bottom of the lake was high (up to a concentration of 10.7 mM). Clone libraries were constructed using the aprA gene, which encodes adenosine-5'-phosphosulfate reductase subunit A, in order to monitor sulfate-reducing bacteria. In the aprA clone libraries, the most abundant sequences were those from the Desulfosarcina-Desulfococcus (DSS) group. A primer set for a DSS group-specific 16S rRNA gene was used to construct another clone library, analysis of which revealed that the uncultured group of sulfate-reducing bacteria, SEEP SRB-1, accounted for nearly half of the obtained sequences. Quantification of the major bacterial groups by catalyzed reporter deposition-fluorescence in situ hybridization demonstrated that the DSS group accounted for 3.2-4.8% of the total bacterial community below the chemocline. The results suggested that the DSS group was one of the major groups of sulfate-reducing bacteria and that these presumably metabolically versatile bacteria might play an important role in sulfur cycling in Lake Harutori. (C) 2014 Elsevier GmbH. All rights reserved.
Type: article (author version)
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小島 久弥

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