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Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake
| Title: | Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake |
| Authors: | Kubo, Kyoko Browse this author →KAKEN DB | | Kojima, Hisaya Browse this author →KAKEN DB | | Fukui, Manabu Browse this author →KAKEN DB |
| Keywords: | Meromictic lake | | Sulfate-reducing bacteria | | aprA | | Sulfide |
| Issue Date: | Oct-2014 |
| Publisher: | Elsevier |
| Journal Title: | Systematic and Applied Microbiology |
| Volume: | 37 |
| Issue: | 7 |
| Start Page: | 510 |
| End Page: | 519 |
| Publisher DOI: | 10.1016/j.syapm.2014.05.008 |
| PMID: | 25034383 |
| Abstract: | The vertical distribution of sulfate-reducing bacteria was investigated in a shallow, eutrophic, meromictic lake, Lake Harutori, located in a residential area of Kushiro, Japan. A steep chemocline, characterized by gradients of oxygen, sulfide and salinity, was found at a depth of 3.5-4.0 m. The sulfide concentration at the bottom of the lake was high (up to a concentration of 10.7 mM). Clone libraries were constructed using the aprA gene, which encodes adenosine-5'-phosphosulfate reductase subunit A, in order to monitor sulfate-reducing bacteria. In the aprA clone libraries, the most abundant sequences were those from the Desulfosarcina-Desulfococcus (DSS) group. A primer set for a DSS group-specific 16S rRNA gene was used to construct another clone library, analysis of which revealed that the uncultured group of sulfate-reducing bacteria, SEEP SRB-1, accounted for nearly half of the obtained sequences. Quantification of the major bacterial groups by catalyzed reporter deposition-fluorescence in situ hybridization demonstrated that the DSS group accounted for 3.2-4.8% of the total bacterial community below the chemocline. The results suggested that the DSS group was one of the major groups of sulfate-reducing bacteria and that these presumably metabolically versatile bacteria might play an important role in sulfur cycling in Lake Harutori. (C) 2014 Elsevier GmbH. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57661 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小島 久弥
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