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Proteomics analysis of heterogeneous flagella in brown algae (Stramenopiles)

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Title: Proteomics analysis of heterogeneous flagella in brown algae (Stramenopiles)
Authors: Fu, Gang Browse this author
Nagasato, Chikako Browse this author →KAKEN DB
Oka, Seiko Browse this author
Cock, J. Mark Browse this author
Motomura, Taizo Browse this author →KAKEN DB
Keywords: Blue light receptor
brown algae
creatine kinase
Issue Date: Sep-2014
Publisher: Elsevier
Journal Title: Protist
Volume: 165
Issue: 5
Start Page: 662
End Page: 675
Publisher DOI: 10.1016/j.protis.2014.07.007
PMID: 25150613
Abstract: Flagella are conserved organelles among eukaryotes and they are composed of many proteins, which are necessary for flagellar assembly, maintenance and function. Stramenopiles, which include brown algae, diatoms and oomycetes, possess two laterally inserted flagella. The anterior flagellum (AF) extends forward and bears tripartite mastigonemes, whilst the smooth posterior flagellum (PF) often has a paraflagellar body structure. These heterogeneous flagella have served as crucial structures in algal studies especially from a viewpoint of phylogeny. However, the protein compositions of the flagella are still largely unknown. Here we report a LC-MS/MS based proteomics analysis of brown algal flagella. In total, 495 flagellar proteins were identified. Functional annotation of the proteome data revealed that brown algal flagellar proteins were associated with cell motility, signal transduction and various metabolic activities. We separately isolated AF and PF and analyzed their protein compositions. This analysis led to the identification of several AF- and PF-specific proteins. Among the PF-specific proteins, we found a candidate novel blue light receptor protein involved in phototaxis, and named it HELMCHROME because of the steering function of PF. Immunological analysis revealed that this protein was localized along the whole length of the PF and concentrated in the paraflagellar body. (C) 2014 Elsevier GmbH. All rights reserved.
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 本村 泰三

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