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A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter

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タイトル: A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter
著者: Kamo, Naoki 著作を一覧する
Hashiba, Tsuyoshi 著作を一覧する
Kikukawa, Takashi 著作を一覧する
Araiso, Tsunehisa 著作を一覧する
Ihara, Kunio 著作を一覧する
Nara, Toshifumi 著作を一覧する
キーワード: Archaeal rhodopsin
Bacteriorhodopsin
Light-driven proton pump
EmrE
Photocycle
SMR
Deltarhodopsin
Archaerhodopsin
Multi-drug resistance transporter
発行日: 2006年 3月10日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 341
号: 2
開始ページ: 285
終了ページ: 290
出版社 DOI: 10.1016/j.bbrc.2005.12.181
抄録: A gene encoding putative retinal protein was cloned from Haloterrigena turkmenica (JCM9743). The deduced amino acid sequence was most closely related to that of deltarhodopsin, which functions as a light-driven H+ pump and was identified in a novel strain Haloterrigena sp. arg-4 (K. Ihara, T. Uemura, I. Katagiri, T. Kitajima-Ihara, Y. Sugiyama, Y. Kimura, Y. Mukohata, Evolution of the archaeal rhodopsins: Evolution rate changes by gene duplication and functional differentiation, J. Mol. Biol. 285 (1999) 163–174. GenBank Accession No. AB009620). Thus, we called the present protein H. turkmenica deltarhodopsin (HtdR) in this report. Differing from the Halobacterium salinarum bacteriorhodopsin (bR), functional expression of HtdR was achieved in Escherichia coli membrane with a high yield of 10–15 mg protein/L culture. The photocycle of purified HtdR was similar to that of bR. The photo-induced electrogenic proton pumping activity of HtdR was verified. We co-expressed both HtdR and EmrE, a proton-coupled multi-drug efflux transporter in E. coli, and the cells successfully extruded ethidium, a substrate of EmrE, on illumination.
Relation (URI): http://www.sciencedirect.com/science/journal/0006291X
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/5787
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加茂 直樹

 

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