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Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene

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Title: Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene
Authors: Han, Xuerong Browse this author
Satoh, Yasuharu Browse this author
Kuriki, Yumi Browse this author
Seino, Teruyuki Browse this author
Fujita, Shinji Browse this author
Suda, Takanori Browse this author
Kobayashi, Takanori Browse this author
Tajima, Kenji Browse this author →KAKEN DB
Keywords: Polyhydroxyalkanoate
Seaweed
Green algae
Ulva
Pha locus
Class I
Massilia
Issue Date: Nov-2014
Publisher: Society for Biotechnology, Japan
Journal Title: Journal of bioscience and bioengineering
Volume: 118
Issue: 5
Start Page: 514
End Page: 519
Publisher DOI: 10.1016/j.jbiosc.2014.04.022
PMID: 24932969
Abstract: We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source. Taxonomic studies and 16S rDNA sequence analysis revealed that strain UMI-21 was phylogenetically related to species of the genus Massilia. The PHA content under the cultivation condition using a 10-L jar fermentor was 45.5% (w/w). This value was higher than that obtained after cultivation in a flask, suggesting the possibility of large-scale PHA production by UMI-21 from starch. A major issue for the industrial production of microbial PHAs is the very high production cost. Starch is a relatively inexpensive substrate that is also found in abundant seaweeds such as Ulva. Therefore, the strain isolated in this study may be very useful for producing PHA from seaweeds containing polysaccharides such as starch. In addition, a 3.7-kbp DNA fragment containing the whole PHA synthase gene (phaC) was obtained from the strain UMI-21. The results of open reading frame (ORF) analysis suggested that the DNA fragment contained two ORFs, which were composed of 1740 (phaC) and 564 bp (phaR). The deduced amino acid sequence of PhaC from strain UMI-21 shared high similarity with PhaC from Ralstonia eutropha, which is a representative PHA-producing bacterium with a class I PHA synthase. This is the first report for the cloning of the PHA synthase gene from Massilia species.
Type: article (author version)
URI: http://hdl.handle.net/2115/57954
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田島 健次

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