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Hokkaido University Collection of Scholarly and Academic Papers >
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Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene
| Title: | Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene |
| Authors: | Han, Xuerong Browse this author | | Satoh, Yasuharu Browse this author | | Kuriki, Yumi Browse this author | | Seino, Teruyuki Browse this author | | Fujita, Shinji Browse this author | | Suda, Takanori Browse this author | | Kobayashi, Takanori Browse this author | | Tajima, Kenji Browse this author →KAKEN DB |
| Keywords: | Polyhydroxyalkanoate | | Seaweed | | Green algae | | Ulva | | Pha locus | | Class I | | Massilia |
| Issue Date: | Nov-2014 |
| Publisher: | Society for Biotechnology, Japan |
| Journal Title: | Journal of bioscience and bioengineering |
| Volume: | 118 |
| Issue: | 5 |
| Start Page: | 514 |
| End Page: | 519 |
| Publisher DOI: | 10.1016/j.jbiosc.2014.04.022 |
| PMID: | 24932969 |
| Abstract: | We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source. Taxonomic studies and 16S rDNA sequence analysis revealed that strain UMI-21 was phylogenetically related to species of the genus Massilia. The PHA content under the cultivation condition using a 10-L jar fermentor was 45.5% (w/w). This value was higher than that obtained after cultivation in a flask, suggesting the possibility of large-scale PHA production by UMI-21 from starch. A major issue for the industrial production of microbial PHAs is the very high production cost. Starch is a relatively inexpensive substrate that is also found in abundant seaweeds such as Ulva. Therefore, the strain isolated in this study may be very useful for producing PHA from seaweeds containing polysaccharides such as starch. In addition, a 3.7-kbp DNA fragment containing the whole PHA synthase gene (phaC) was obtained from the strain UMI-21. The results of open reading frame (ORF) analysis suggested that the DNA fragment contained two ORFs, which were composed of 1740 (phaC) and 564 bp (phaR). The deduced amino acid sequence of PhaC from strain UMI-21 shared high similarity with PhaC from Ralstonia eutropha, which is a representative PHA-producing bacterium with a class I PHA synthase. This is the first report for the cloning of the PHA synthase gene from Massilia species. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57954 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田島 健次
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