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Nitric Oxide Produced During Sublethal Ischemia Is Crucial for the Preconditioning-Induced Down-Regulation of Glutamate Transporter GLT-1 in Neuron/Astrocyte Co-Cultures

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タイトル: Nitric Oxide Produced During Sublethal Ischemia Is Crucial for the Preconditioning-Induced Down-Regulation of Glutamate Transporter GLT-1 in Neuron/Astrocyte Co-Cultures
著者: Yamada, Takeshi 著作を一覧する
Kawahara, Koichi 著作を一覧する
Kosugi, Tatsuro 著作を一覧する
Tanaka, Motoki 著作を一覧する
キーワード: Nitric oxide
GLT-1
preconditioning
ischemic tolerance
発行日: 2006年 1月
出版者: Springer
誌名: Neurochemical Research
巻: 31
号: 1
開始ページ: 49
終了ページ: 56
出版社 DOI: 10.1007/s11064-005-9077-4
抄録: In the brain, prior sublethal ischemia (preconditioning, PC) produces tolerance of neurons to subsequent lethal ischemia. This study aims at elucidating whether and how nitric oxide (NO) produced during PC is involved in the PC-induced ischemic tolerance of neurons in neuron/astrocyte co-cultures. The rise in the extracellular concentration of glutamate during ischemia caused by the reversed uptake of glutamate (Glu) by the astrocytic Glu transporter GLT-1 was markedly suppressed by the prior PC treatment, but the suppression was reversed by treatment with an inhibitor of nitric oxide synthase (NOS) during PC. Immunocytochemical and Western blot analyses demonstrated that the expression of GLT-1 was down-regulated after the PC insult, and this down-regulation was also antagonized by treatment with NOS inhibitors during PC. Here we show that nNOS-derived NO produced during PC was crucial for the down-regulation of astrocytic GLT-1, and this down-regulation coincided with an increased survival rate of neurons.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/5814
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 河原 剛一

 

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