|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle
| Title: | Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle |
| Authors: | Nagatomo, H. Browse this author | | Kagawa, S. Browse this author | | Kishi, Y. Browse this author | | Takuma, T. Browse this author | | Sada, A. Browse this author | | Yamanaka, K.-i. Browse this author | | Abe, Y. Browse this author | | Wada, Y. Browse this author | | Takahashi, M. Browse this author | | Kono, T. Browse this author | | Kawahara, M. Browse this author →KAKEN DB |
| Keywords: | bovine | | blastocyst | | cell lineage specification | | gene expression |
| Issue Date: | 1-Jan-2013 |
| Publisher: | the Society for the Study of Reproduction |
| Journal Title: | Biology of Reproduction |
| Volume: | 88 |
| Issue: | 6 |
| Start Page: | 158 |
| Publisher DOI: | 10.1095/biolreprod.113.108993 |
| PMID: | 23677984 |
| Abstract: | Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding reliable transcriptional networks that orchestrate complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative RT-PCR and in situ hybridization using the site-specific cell samples. As a result, we identified 7 ICM-dominant genes and 5 TE-dominant genes not yet found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the preimplantation bovine embryo. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58264 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 川原 学
|
