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Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle

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Title: Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle
Authors: Nagatomo, H. Browse this author
Kagawa, S. Browse this author
Kishi, Y. Browse this author
Takuma, T. Browse this author
Sada, A. Browse this author
Yamanaka, K.-i. Browse this author
Abe, Y. Browse this author
Wada, Y. Browse this author
Takahashi, M. Browse this author
Kono, T. Browse this author
Kawahara, M. Browse this author →KAKEN DB
Keywords: bovine
cell lineage specification
gene expression
Issue Date: 1-Jan-2013
Publisher: the Society for the Study of Reproduction
Journal Title: Biology of Reproduction
Volume: 88
Issue: 6
Start Page: 158
Publisher DOI: 10.1095/biolreprod.113.108993
PMID: 23677984
Abstract: Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding reliable transcriptional networks that orchestrate complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative RT-PCR and in situ hybridization using the site-specific cell samples. As a result, we identified 7 ICM-dominant genes and 5 TE-dominant genes not yet found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the preimplantation bovine embryo.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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