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Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids
| Title: | Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids |
| Authors: | Kondo, Natsuki Browse this author | | Ohno, Yusuke Browse this author →KAKEN DB | | Yamagata, Maki Browse this author | | Obara, Takashi Browse this author | | Seki, Naoya Browse this author | | Kitamura, Takuya Browse this author | | Naganuma, Tatsuro Browse this author | | Kihara, Akio Browse this author →KAKEN DB |
| Issue Date: | Oct-2014 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Nature communications |
| Volume: | 5 |
| Start Page: | 5338 |
| Publisher DOI: | 10.1038/ncomms6338 |
| PMID: | 25345524 |
| Abstract: | The long-chain base phytosphingosine is a component of sphingolipids and exists in yeast, plants and some mammalian tissues. Phytosphingosine is unique in that it possesses an additional hydroxyl group compared with other long-chain bases. However, its metabolism is unknown. Here we show that phytosphingosine is metabolized to odd-numbered fatty acids and is incorporated into glycerophospholipids both in yeast and mammalian cells. Disruption of the yeast gene encoding long-chain base 1-phosphate lyase, which catalyzes the committed step in the metabolism of phytosphingosine to glycerophospholipids, causes an similar to 40% reduction in the level of phosphatidylcholines that contain a C15 fatty acid. We also find that 2-hydroxypalmitic acid is an intermediate of the phytosphingosine metabolic pathway. Furthermore, we show that the yeast MPO1 gene, whose product belongs to a large, conserved protein family of unknown function, is involved in phytosphingosine metabolism. Our findings provide insights into fatty acid diversity and identify a pathway by which hydroxyl group-containing lipids are metabolized. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58535 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木原 章雄
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