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Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids

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Title: Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids
Authors: Kondo, Natsuki Browse this author
Ohno, Yusuke Browse this author →KAKEN DB
Yamagata, Maki Browse this author
Obara, Takashi Browse this author
Seki, Naoya Browse this author
Kitamura, Takuya Browse this author
Naganuma, Tatsuro Browse this author
Kihara, Akio Browse this author →KAKEN DB
Issue Date: Oct-2014
Publisher: Nature Publishing Group
Journal Title: Nature communications
Volume: 5
Start Page: 5338
Publisher DOI: 10.1038/ncomms6338
PMID: 25345524
Abstract: The long-chain base phytosphingosine is a component of sphingolipids and exists in yeast, plants and some mammalian tissues. Phytosphingosine is unique in that it possesses an additional hydroxyl group compared with other long-chain bases. However, its metabolism is unknown. Here we show that phytosphingosine is metabolized to odd-numbered fatty acids and is incorporated into glycerophospholipids both in yeast and mammalian cells. Disruption of the yeast gene encoding long-chain base 1-phosphate lyase, which catalyzes the committed step in the metabolism of phytosphingosine to glycerophospholipids, causes an similar to 40% reduction in the level of phosphatidylcholines that contain a C15 fatty acid. We also find that 2-hydroxypalmitic acid is an intermediate of the phytosphingosine metabolic pathway. Furthermore, we show that the yeast MPO1 gene, whose product belongs to a large, conserved protein family of unknown function, is involved in phytosphingosine metabolism. Our findings provide insights into fatty acid diversity and identify a pathway by which hydroxyl group-containing lipids are metabolized.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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