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Non-linear pharmacodynamics in a non-viral gene delivery system: Positive non-linear relationship between dose and transfection efficiency
Title: | Non-linear pharmacodynamics in a non-viral gene delivery system: Positive non-linear relationship between dose and transfection efficiency |
Authors: | Moriguchi, Rumiko Browse this author | Kogure, Kentaro Browse this author | Iwasa, Akitada Browse this author | Akita, Hidetaka Browse this author | Hideyoshi, Harashima5 Browse this author →KAKEN DB |
Authors(alt): | 原島, 秀吉5 |
Keywords: | non-viral gene delivery system | intracellular pharmacokinetics | pharmacodinamics | synergism |
Issue Date: | Feb-2006 |
Publisher: | Elsevier B.V |
Journal Title: | Journal of Controlled Release |
Volume: | 110 |
Issue: | 3 |
Start Page: | 605 |
End Page: | 609 |
Publisher DOI: | 10.1016/j.jconrel.2005.10.021 |
PMID: | 16360229 |
Abstract: | A remarkable non-linearity was found between dose and transfection activities of non-viral gene delivery systems, such as a Lipofectamine/DNA complex and an octaarginine-modified multifunctional envelope-type nano device (R8-MEND). We measured the nuclear delivery of pDNA to distinguish the non-linearity in intracellular pharmacokinetics or pharmacodynamics after transfection with R8-MEND at different doses. A remarkable positive non-linearity was found in the pharmacodynamics when the dose was increased. Even dummy pDNA enhanced the efficiency of transcription and/or translation per pDNA in the nucleus, but empty liposomes did not. These results suggest the importance of controlled pharmacodynamics as well as the importance of intracellular pharmacokinetics for the rational design of non-viral gene delivery systems. |
Description: | http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/description |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/5922 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 原島 秀吉
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