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Functional Characterization of 5-Oxoproline Transport via SLC16A1/MCT1
| Title: | Functional Characterization of 5-Oxoproline Transport via SLC16A1/MCT1 |
| Authors: | Sasaki, Shotaro Browse this author | | Futagi, Yuya Browse this author | | Kobayashi, Masaki Browse this author →KAKEN DB | | Ogura, Jiro Browse this author →KAKEN DB | | Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | transporter | | proton transport | | Xenopus | | brain | | single-nucleotide polymorphism |
| Issue Date: | 23-Jan-2015 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 290 |
| Issue: | 4 |
| Start Page: | 2303 |
| End Page: | 2311 |
| Publisher DOI: | 10.1074/jbc.M114.581892 |
| Abstract: | Thyrotropin-releasing hormone is a tripeptide that consists of 5-oxoproline, histidine, and proline. The peptide is rapidly metabolized by various enzymes. 5-Oxoproline is produced by enzymatic hydrolysis in a variety of peptides. Previous studies showed that 5-oxoproline could become a possible biomarker for autism spectrum disorders. Here we demonstrate the involvement of SLC16A1 in the transport of 5-oxoproline. An SLC16A1 polymorphism (rs1049434) was recently identified. However, there is no information about the effect of the polymorphism on SLC16A1 function. In this study, the polymorphism caused an observable change in 5-oxoproline and lactate transport via SLC16A1. The Michaelis constant (K-m) was increased in an SLC16A1 mutant compared with that in the wild type. In addition, the proton concentration required to produce half-maximal activation of transport activity (K-0.5, H(+)) was increased in the SLC16A1 mutant compared with that in the wild type. Furthermore, we examined the transport of 5-oxoproline in T98G cells as an astrocyte cell model. Despite the fact that 5-oxoproline is an amino acid derivative, Na+-dependent and amino acid transport systems scarcely contributed to 5-oxoproline transport. Based on our findings, we conclude that H+-coupled 5-oxoproline transport is mediated solely by SLC16A1 in the cells. |
| Rights: | This research was originally published in Journal of Biological Chemistry. Shotaro Sasaki, Yuya Futagi, Masaki Kobayashi, Jiro Ogura, and Ken Iseki. Functional Characterization of 5-Oxoproline Transport via SLC16A1/MCT1. Journal of Biological Chemistry. 2015; 290(4):2303-2311. © the American Society for Biochemistry and Molecular Biology. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/59282 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 井関 健
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