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Hokkaido University Collection of Scholarly and Academic Papers >
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Reversed operation of glutamate transporter GLT-1 is crucial to the development of preconditioning-induced ischemic tolerance of neurons in neuron/astrocyte co-cultures
| Title: | Reversed operation of glutamate transporter GLT-1 is crucial to the development of preconditioning-induced ischemic tolerance of neurons in neuron/astrocyte co-cultures |
| Authors: | Kawahara, Koichi1 Browse this author →KAKEN DB | | Kosugi, Tatsuro Browse this author | | Tanaka, Motoki Browse this author | | Nakajima, Takayuki Browse this author | | Yamada, Takeshi Browse this author |
| Authors(alt): | 河原, 剛一1 |
| Keywords: | GLT-1 | | preconditioning | | neuronal ischemic tolerance |
| Issue Date: | Feb-2005 |
| Publisher: | Wiley-Liss, Inc. |
| Journal Title: | Glia |
| Volume: | 49 |
| Issue: | 3 |
| Start Page: | 349 |
| End Page: | 359 |
| Publisher DOI: | 10.1002/glia.20114 |
| PMID: | 15538756 |
| Abstract: | Sublethal ischemia leads to increased tolerance against subsequent prolonged cerebral ischemia in vivo. In the present study, we investigated the roles of the astrocytic glutamate (Glu) transporter GLT-1 in preconditioning (PC)-induced neuronal ischemic tolerance in cortical neuron/astrocyte co-cultures. Ischemia in vitro was simulated by subjecting cultures to both oxygen and glucose deprivation (OGD). A sublethal OGD (PC) increased the survival rate of neurons significantly when cultures were exposed to a lethal OGD 24 h later. The extracellular concentration of Glu increased significantly during PC, and treatment with an inhibitor of N-methyl-D-actetate (NMDA) receptors significantly reversed the PC-induced ischemic tolerance of neurons, suggesting that the increase in extracellular concentration of Glu during PC was critical to the development of PC-induced neuronal ischemic tolerance via the activation of NMDA receptors. Treatment with a GLT-1 blocker during PC suppressed this increase in Glu significantly, and antagonized the PC-induced neuronal ischemic tolerance. This study suggested that the reversed operation of GLT-1 was crucial to the development of neuronal ischemic tolerance. |
| Rights: | Copyright © 2005 John Wiley & Sons, Inc., Glia, Vol.49-3, p. 349-359 |
| Relation: | http://www.interscience.wiley.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/5931 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 河原 剛一
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