HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Research Institute for Electronic Science >
Peer-reviewed Journal Articles, etc >

Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser

Files in This Item:
31_307-1.pdf1.06 MBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser
Authors: Otomo, Kohei Browse this author
Hibi, Terumasa Browse this author →KAKEN DB
Murata, Takashi Browse this author →KAKEN DB
Watanabe, Hirotaka Browse this author
Kawakami, Ryosuke Browse this author →KAKEN DB
Nakayama, Hiroshi Browse this author
Hasebe, Mitsuyasu Browse this author →KAKEN DB
Nemoto, Tomomi Browse this author →KAKEN DB
Keywords: Two-photon excitation microscopy
multi-point laser scanning method
confocal microscopy
Yb-based laser
Issue Date: 10-Apr-2015
Publisher: Japan Society for Analytical Chemistry
Journal Title: Analytical sciences
Volume: 31
Issue: 4
Start Page: 307
End Page: 313
Publisher DOI: 10.2116/analsci.31.307
PMID: 25864674
Abstract: The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 根本 知己

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University