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Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser
| Title: | Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser |
| Authors: | Otomo, Kohei Browse this author | | Hibi, Terumasa Browse this author →KAKEN DB | | Murata, Takashi Browse this author →KAKEN DB | | Watanabe, Hirotaka Browse this author | | Kawakami, Ryosuke Browse this author →KAKEN DB | | Nakayama, Hiroshi Browse this author | | Hasebe, Mitsuyasu Browse this author →KAKEN DB | | Nemoto, Tomomi Browse this author →KAKEN DB |
| Keywords: | Two-photon excitation microscopy | | multi-point laser scanning method | | confocal microscopy | | Yb-based laser |
| Issue Date: | 10-Apr-2015 |
| Publisher: | Japan Society for Analytical Chemistry |
| Journal Title: | Analytical sciences |
| Volume: | 31 |
| Issue: | 4 |
| Start Page: | 307 |
| End Page: | 313 |
| Publisher DOI: | 10.2116/analsci.31.307 |
| PMID: | 25864674 |
| Abstract: | The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59325 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 根本 知己
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