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Three-dimensional imaging of biological cells with picosecond ultrasonics
| Title: | Three-dimensional imaging of biological cells with picosecond ultrasonics |
| Authors: | Danworaphong, Sorasak Browse this author | | Tomoda, Motonobu Browse this author →KAKEN DB | | Matsumoto, Yuki Browse this author | | Matsuda, Osamu Browse this author →KAKEN DB | | Ohashi, Toshiro Browse this author →KAKEN DB | | Watanabe, Hiromu Browse this author | | Nagayama, Masafumi Browse this author →KAKEN DB | | Gohara, Kazutoshi Browse this author →KAKEN DB | | Otsuka, Paul H. Browse this author | | Wright, Oliver B. Browse this author →KAKEN DB |
| Issue Date: | 21-Apr-2015 |
| Publisher: | American Institute of Physics |
| Journal Title: | Applied physics letters |
| Volume: | 106 |
| Issue: | 16 |
| Start Page: | 163701 |
| Publisher DOI: | 10.1063/1.4918275 |
| Abstract: | We use picosecond ultrasonics to image animal cells in vitro-a bovine aortic endothelial cell and a mouse adipose cell-fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with similar to 1 mu m lateral and similar to 150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli. |
| Rights: | Copyright 2015 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 106, 163701, 2015, and may be found at http://dx.doi.org/10.1063/1.4918275 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59330 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: WRIGHT O. B.
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