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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats
| Title: | Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats |
| Authors: | Iwabuchi, Sadahiro Browse this author | | Kawahara, Koichi2 Browse this author →KAKEN DB | | Makisaka, Koji Browse this author | | Sato, Hideomi Browse this author |
| Authors(alt): | 河原, 剛一2 |
| Keywords: | Calcium waves | | Caged ionophore | | Photolysis | | Astrocytes | | Gap junction |
| Issue Date: | Sep-2002 |
| Publisher: | Springer Berlin / Heidelberg |
| Journal Title: | Experimental Brain Research |
| Volume: | 146 |
| Issue: | 1 |
| Start Page: | 103 |
| End Page: | 116 |
| Publisher DOI: | 10.1007/s00221-002-1149-y |
| PMID: | 12192584 |
| Abstract: | Waves of elevated intracellular free calcium that propagate between neighboring astrocytes are important for the intercellular communication between astrocytes as well as between neurons and astrocytes. However, the mechanisms responsible for the initiation and propagation of astrocytic calcium waves remain unclear. In this study, intercellular calcium waves were evoked by focal photolysis of a caged calcium ionophore (DMNPE-caged Br A23187) in cultured astrocytes from newborn rats. The focal photolysis of the caged compound resulted in the increase in intracellular calcium in a single astrocyte, and this increase then propagated to neighboring astrocytes. We also analyzed the spatiotemporal characteristics of the intercellular calcium waves, and estimated the propagation pathways for them. The method using a caged calcium ionophore described in this study provides a new in vitro model for the analysis of intercellular calcium waves. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/5938 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 河原 剛一
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