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Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/5938

Title: Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats
Authors: Iwabuchi, Sadahiro Browse this author
Kawahara, Koichi2 Browse this author →KAKEN DB
Makisaka, Koji Browse this author
Sato, Hideomi Browse this author
Authors(alt): 河原, 剛一2
Keywords: Calcium waves
Caged ionophore
Photolysis
Astrocytes
Gap junction
Issue Date: Sep-2002
Publisher: Springer Berlin / Heidelberg
Journal Title: Experimental Brain Research
Volume: 146
Issue: 1
Start Page: 103
End Page: 116
Publisher DOI: 10.1007/s00221-002-1149-y
PMID: 12192584
Abstract: Waves of elevated intracellular free calcium that propagate between neighboring astrocytes are important for the intercellular communication between astrocytes as well as between neurons and astrocytes. However, the mechanisms responsible for the initiation and propagation of astrocytic calcium waves remain unclear. In this study, intercellular calcium waves were evoked by focal photolysis of a caged calcium ionophore (DMNPE-caged Br A23187) in cultured astrocytes from newborn rats. The focal photolysis of the caged compound resulted in the increase in intracellular calcium in a single astrocyte, and this increase then propagated to neighboring astrocytes. We also analyzed the spatiotemporal characteristics of the intercellular calcium waves, and estimated the propagation pathways for them. The method using a caged calcium ionophore described in this study provides a new in vitro model for the analysis of intercellular calcium waves.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/5938
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 河原 剛一

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