|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds
| Title: | Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds |
| Authors: | HASHIMOTO, Masanori Browse this author | | TOSHIMA, Hirokazu Browse this author | | YONEZAWA, Tetsu Browse this author →KAKEN DB | | KAWAI, Koji Browse this author | | NARUSHIMA, Takashi Browse this author | | KAGA, Masayuki Browse this author | | ENDO, Kazuhiko Browse this author |
| Keywords: | Ag-NPs | | Biocompatibility | | Endocytosis | | SEM | | TEM |
| Issue Date: | 2013 |
| Publisher: | Japanese Society for Dental Materials and Devices |
| Journal Title: | Dental Materials Journal |
| Volume: | 32 |
| Issue: | 5 |
| Start Page: | 725 |
| End Page: | 733 |
| Publisher DOI: | 10.4012/dmj.2012-203 |
| Abstract: | With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new water-dispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured marine macrophages (RAW264.7) and osteoblast-like cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59713 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 米澤 徹
|
