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Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

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Title: Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds
Authors: HASHIMOTO, Masanori Browse this author
TOSHIMA, Hirokazu Browse this author
YONEZAWA, Tetsu Browse this author →KAKEN DB
KAWAI, Koji Browse this author
NARUSHIMA, Takashi Browse this author
KAGA, Masayuki Browse this author
ENDO, Kazuhiko Browse this author
Keywords: Ag-NPs
Biocompatibility
Endocytosis
SEM
TEM
Issue Date: 2013
Publisher: Japanese Society for Dental Materials and Devices
Journal Title: Dental Materials Journal
Volume: 32
Issue: 5
Start Page: 725
End Page: 733
Publisher DOI: 10.4012/dmj.2012-203
Abstract: With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new water-dispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured marine macrophages (RAW264.7) and osteoblast-like cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs.
Type: article
URI: http://hdl.handle.net/2115/59713
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 米澤 徹

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