| Title: | Identification of DJ-1-Associated Regions on Human Genes from SH-SY5Y Cells using Chromatin Immunoprecipitation Sequence Technique |
| Authors: | Yamane, Takuya Browse this author |
| Sugimoto, Naoyuki Browse this author |
| Maita, Hiroshi Browse this author →KAKEN DB |
| Watanabe, Kazufumi Browse this author |
| Takahashi-Niki, Kazuko Browse this author →KAKEN DB |
| Maita, Chinatsu Browse this author |
| Kato-Ose, Izumi Browse this author |
| Ishikawa, Shizuma Browse this author |
| Gao, Jian-wei Browse this author |
| Kitaura, Hirotake Browse this author →KAKEN DB |
| Niki, Takeshi Browse this author →KAKEN DB |
| Iguchi-Ariga, Sanae MM Browse this author →KAKEN DB |
| Ariga, Hiroyoshi Browse this author →KAKEN DB |
| Keywords: | ChIP sequences |
| DJ-1 |
| Transcriptional regulation |
| Oxidative stress |
| Genome-wide analysis |
| Cell growth |
| Issue Date: | 29-Nov-2013 |
| Publisher: | OMICS International |
| Journal Title: | Molecular Biology |
| Volume: | 3 |
| Issue: | 1 |
| Start Page: | Page 1 of 6 |
| End Page: | Page 6 of 6 |
| Publisher DOI: | 10.4172/2168-9547.1000115 |
| Abstract: | DJ-1, a cancer- and Parkinson’s disease-associated protein, works as a coactivator to various transcription factors. In this study, DNA fragments that bind to DJ-1 complexes were obtained by a chromatin immunoprecipitation sequencing with an anti-human DJ-1 antibody using chromatin from SH-SY5Y cells. We identified 60 different sequences as potential DJ-1 complex-binding sites in genes. Of sequences identified, expression levels of DJ-1-associated sitecontaining genes for DNA polymerase N, estrogen receptor α and S-adenosylhomocysteine hydrolase like-2 were decreased in DJ-1-knockdown cells and in 6-OHDA-treated cells. These studies suggest that DJ-1 regulates the expression of versatile genes at the transcriptional level and that some of the genes are regulated by DJ-1 in an oxidative status-dependent manner |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59720 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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