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Identification of DJ-1-Associated Regions on Human Genes from SH-SY5Y Cells using Chromatin Immunoprecipitation Sequence Technique

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Title: Identification of DJ-1-Associated Regions on Human Genes from SH-SY5Y Cells using Chromatin Immunoprecipitation Sequence Technique
Authors: Yamane, Takuya Browse this author
Sugimoto, Naoyuki Browse this author
Maita, Hiroshi Browse this author →KAKEN DB
Watanabe, Kazufumi Browse this author
Takahashi-Niki, Kazuko Browse this author →KAKEN DB
Maita, Chinatsu Browse this author
Kato-Ose, Izumi Browse this author
Ishikawa, Shizuma Browse this author
Gao, Jian-wei Browse this author
Kitaura, Hirotake Browse this author →KAKEN DB
Niki, Takeshi Browse this author →KAKEN DB
Iguchi-Ariga, Sanae MM Browse this author →KAKEN DB
Ariga, Hiroyoshi Browse this author →KAKEN DB
Keywords: ChIP sequences
Transcriptional regulation
Oxidative stress
Genome-wide analysis
Cell growth
Issue Date: 29-Nov-2013
Publisher: OMICS International
Journal Title: Molecular Biology
Volume: 3
Issue: 1
Start Page: Page 1 of 6
End Page: Page 6 of 6
Publisher DOI: 10.4172/2168-9547.1000115
Abstract: DJ-1, a cancer- and Parkinson’s disease-associated protein, works as a coactivator to various transcription factors. In this study, DNA fragments that bind to DJ-1 complexes were obtained by a chromatin immunoprecipitation sequencing with an anti-human DJ-1 antibody using chromatin from SH-SY5Y cells. We identified 60 different sequences as potential DJ-1 complex-binding sites in genes. Of sequences identified, expression levels of DJ-1-associated sitecontaining genes for DNA polymerase N, estrogen receptor α and S-adenosylhomocysteine hydrolase like-2 were decreased in DJ-1-knockdown cells and in 6-OHDA-treated cells. These studies suggest that DJ-1 regulates the expression of versatile genes at the transcriptional level and that some of the genes are regulated by DJ-1 in an oxidative status-dependent manner
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山根 拓也

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