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Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.

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Title: Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.
Authors: Sasaki, Michihito Browse this author →KAKEN DB
Hasebe, Rie Browse this author →KAKEN DB
Makino, Yoshinori Browse this author →KAKEN DB
Suzuki, Tadaki Browse this author →KAKEN DB
Fukushi, Hideto Browse this author →KAKEN DB
Okamoto, Minoru Browse this author →KAKEN DB
Matsuda, Kazuya Browse this author
Taniyama, Hiroyuki Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Kimura, Takashi Browse this author →KAKEN DB
Issue Date: Apr-2011
Publisher: Wiley-Blackwell
Journal Title: Genes to Cells
Volume: 16
Issue: 4
Start Page: 343
End Page: 357
Publisher DOI: 10.1111/j.1365-2443.2011.01491.x
PMID: 21306483
Abstract: The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells.
Type: article
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐々木 道仁

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