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Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.
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| Title: | Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. |
| Authors: | Sasaki, Michihito Browse this author →KAKEN DB | | Hasebe, Rie Browse this author →KAKEN DB | | Makino, Yoshinori Browse this author →KAKEN DB | | Suzuki, Tadaki Browse this author →KAKEN DB | | Fukushi, Hideto Browse this author →KAKEN DB | | Okamoto, Minoru Browse this author →KAKEN DB | | Matsuda, Kazuya Browse this author | | Taniyama, Hiroyuki Browse this author →KAKEN DB | | Sawa, Hirofumi Browse this author →KAKEN DB | | Kimura, Takashi Browse this author →KAKEN DB |
| Issue Date: | Apr-2011 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Genes to Cells |
| Volume: | 16 |
| Issue: | 4 |
| Start Page: | 343 |
| End Page: | 357 |
| Publisher DOI: | 10.1111/j.1365-2443.2011.01491.x |
| PMID: | 21306483 |
| Abstract: | The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells. |
| Rights: | https://creativecommons.org/licenses/by-nc-sa/4.0/deed.ja |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59847 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐々木 道仁
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