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Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli

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タイトル: Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli
著者: Kadoya, Ryosuke 著作を一覧する
Kodama, Yu 著作を一覧する
Matsumoto, Ken'ichiro 著作を一覧する
Taguchi, Seiichi 著作を一覧する
キーワード: biobased plastic
Escherichia coli
polyhydroxyalkanoate
polyhydroxybutyrate
poly(lactic acid)
sigma factor
発行日: 2015年
出版者: Taylor & Francis
誌名: Bioengineered
巻: 6
号: 5
開始ページ: 307
終了ページ: 311
出版社 DOI: 10.1080/21655979.2015.1069449
抄録: The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4 disrupting mutants of sigma factors in Escherichia coli, rpoN, rpoS, fliA, fecI, were used as platforms for production of P(LA-co-3HB), increases in the production level and LA fraction of the copolymer were observed for the mutant strain with rpoN disruption. These positive impacts on the polymer production were caused in an indirect manner via changes in the multiple genes governed by RpoN. A genome-wide engineering by sigma factors would be a versatile approach for the production of value-added products of interest and available for combination with the other beneficial tools.
Rights: ©2015 Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, and Seiichi Taguchi. Published with license by Taylor & Francis Group, LLC.
資料タイプ: article
URI: http://hdl.handle.net/2115/60235
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田口 精一

 

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