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Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli

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Title: Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli
Authors: Kadoya, Ryosuke Browse this author
Kodama, Yu Browse this author
Matsumoto, Ken'ichiro Browse this author →KAKEN DB
Taguchi, Seiichi Browse this author →KAKEN DB
Keywords: biobased plastic
Escherichia coli
poly(lactic acid)
sigma factor
Issue Date: 2015
Publisher: Taylor & Francis
Journal Title: Bioengineered
Volume: 6
Issue: 5
Start Page: 307
End Page: 311
Publisher DOI: 10.1080/21655979.2015.1069449
Abstract: The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4 disrupting mutants of sigma factors in Escherichia coli, rpoN, rpoS, fliA, fecI, were used as platforms for production of P(LA-co-3HB), increases in the production level and LA fraction of the copolymer were observed for the mutant strain with rpoN disruption. These positive impacts on the polymer production were caused in an indirect manner via changes in the multiple genes governed by RpoN. A genome-wide engineering by sigma factors would be a versatile approach for the production of value-added products of interest and available for combination with the other beneficial tools.
Rights: ©2015 Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, and Seiichi Taguchi. Published with license by Taylor & Francis Group, LLC.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田口 精一

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