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Contrasting allelic distribution of CO/Hd1 homologues in Miscanthus sinensis from the East Asian mainland and the Japanese archipelago

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Title: Contrasting allelic distribution of CO/Hd1 homologues in Miscanthus sinensis from the East Asian mainland and the Japanese archipelago
Authors: Nagano, Hironori Browse this author
Clark, Lindsay V. Browse this author
Zhao, Hua Browse this author
Peng, Junhua Browse this author
Yoo, Ji Hye Browse this author
Heo, Kweon Browse this author
Yu, Chang Yeon Browse this author
Anzoua, Kossonou Guillaume Browse this author
Matsuo, Tomoaki Browse this author
Sacks, Erik J. Browse this author
Yamada, Toshihiko Browse this author →KAKEN DB
Keywords: Bioenergy
flowering time gene
gene duplication
geographical allelic differentiation
Miscanthus sinensis
MITEs
Issue Date: 18-Jun-2015
Publisher: Oxford University Press
Journal Title: Journal of experimental botany
Volume: 66
Issue: 14
Start Page: 4227
End Page: 4237
Publisher DOI: 10.1093/jxb/erv292
Abstract: The genus Miscanthus is a perennial C-4 grass native to eastern Asia and is a promising candidate bioenergy crop for cool temperate areas. Flowering time is a crucial factor governing regional and seasonal adaptation; in addition, it is also a key target trait for extending the vegetative phase to improve biomass potential. Homologues of CONSTANS (CO)/Heading date 1(Hd1) were cloned from Miscanthus sinensis and named MsiHd1. Sequences of MsiHd1 homologues were compared among 24 wild M. sinensis accessions from Japan, 14 from China, and three from South Korea. Two to five MsiHd1 alleles in each accession were identified, suggesting that MsiHd1 consists of at least three loci in the Miscanthus genome. Verifying the open reading frame in MsiHd1, they were classified as putative functional alleles without mutations or non-functional alleles caused by indels. The Neighbor-Joining tree indicated that one of the multiple MsiHd1 loci is a pseudogene locus without any functional alleles. The pseudogene locus was named MsiHd1b, and the other loci were considered to be part of the MsiHd1a multi-locus family. Interestingly, in most Japanese accessions 50% or more of the MsiHd1a alleles were non-functional, whereas accessions from the East Asian mainland harboured only functional alleles. Five novel miniature inverted transposable elements (MITEs) (MsiMITE1-MsiMITE5) were observed in MsiHd1a/b. MsiMITE1, detected in exon 1 of MsiHd1a, was only observed in Japanese accessions and its revertant alleles derived from retransposition were predominantly in Chinese accessions. These differences in MsiHd1a show that the dependency on functional MsiHd1a alleles is different between accessions from the East Asian mainland and Japan.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [Journal of Experimental Botany] following peer review. The definitive publisher-authenticated version [Vol. 66, No. 14 pp. 4227–4237] is available online at:doi:10.1093/jxb/erv292.
http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/60253
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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