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Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification

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Title: Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification
Authors: Kitamura, Takuya Browse this author
Takagi, Shuyu Browse this author
Naganuma, Tatsuro Browse this author
Kihara, Akio Browse this author →KAKEN DB
Keywords: aldehyde dehydrogenase
lipid
lipid droplet
lipid modification
prenylation
protein targeting
Issue Date: 2-Jan-2015
Publisher: Portland Press Ltd
Journal Title: Biochemical journal
Volume: 465
Start Page: 79
End Page: 87
Publisher DOI: 10.1042/BJ20140624
PMID: 25286108
Abstract: Aldehyde dehydrogenases (ALDHs) catalyse the conversion of toxic aldehydes into non-toxic carboxylic acids. Of the 21 ALDHs in mice, it is the ALDH3 family members (ALDH3A1, ALDH3A2, ALDH3B1, ALDH3B2 and ALDH3B3) that are responsible for the removal of lipid-derived aldehydes. However, ALDH3B2 and ALDH3B3 have yet to be characterized. In the present study, we examined the enzyme activity, tissue distribution and subcellular localization of ALDH3B2 and ALDH3B3. Both were found to exhibit broad substrate preferences from medium to long-chain aldehydes, resembling ALDH3A2 and ALDH3B1. Although ALDH3B2 and ALDH3B3 share extremely high sequence similarity, their localizations differ, with ALDH3B2 found in lipid droplets and ALDH3B3 localized to the plasma membrane. Both were modified by prenylation at their C-termini; this modification greatly influenced their membrane localization and enzymatic activity towards hexadecanal. We found that their C-terminal regions, particularly the two tryptophan residues (Trp(462) and Trp(469)) of ALDH3B2 and the two arginine residues (Arg(462) and Arg(463)) of ALDH3B3, were important for the determination of their specific localization. Abnormal quantity and perhaps quality of lipid droplets are implicated in several metabolic diseases. We speculate that ALDH3B2 acts to remove lipid-derived aldehydes in lipid droplets generated via oxidative stress as a quality control mechanism.
Rights: The Version of Record (VoR) is available at www.biochemj.org
Type: article (author version)
URI: http://hdl.handle.net/2115/60410
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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