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The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition

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Title: The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition
Authors: Nishino, Kanako Browse this author
Obara, Keisuke Browse this author →KAKEN DB
Kihara, Akio Browse this author →KAKEN DB
Keywords: lipid bilayer
plasma membrane
yeast
phospholipid
signal transduction
lipid asymmetry
alkaline response
Rim21
Issue Date: 25-Dec-2015
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: Journal of Biological Chemistry
Volume: 290
Issue: 52
Start Page: 30797
End Page: 30805
Publisher DOI: 10.1074/jbc.M115.674382
Abstract: Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions, but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an “antenna hypothesis”, in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
Rights: This research was originally published in Journal of Biological Chemistry. Kanako Nishino, Keisuke Obara and Akio Kihara. The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: Insights into Sensing Mechanisms for Plasma Membrane Lipid Asymmetry. Journal of Biological Chemistry. 2015; Vol.290:30797-30805. ©the American Society for Biochemistry and Molecular Biology.
Type: article (author version)
URI: http://hdl.handle.net/2115/60457
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小原 圭介

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