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The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition

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タイトル: The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition
著者: Nishino, Kanako 著作を一覧する
Obara, Keisuke 著作を一覧する
Kihara, Akio 著作を一覧する
キーワード: lipid bilayer
plasma membrane
yeast
phospholipid
signal transduction
lipid asymmetry
alkaline response
Rim21
発行日: 2015年12月25日
出版者: American Society for Biochemistry and Molecular Biology
誌名: Journal of Biological Chemistry
巻: 290
号: 52
開始ページ: 30797
終了ページ: 30805
出版社 DOI: 10.1074/jbc.M115.674382
抄録: Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions, but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an “antenna hypothesis”, in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
Rights: This research was originally published in Journal of Biological Chemistry. Kanako Nishino, Keisuke Obara and Akio Kihara. The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: Insights into Sensing Mechanisms for Plasma Membrane Lipid Asymmetry. Journal of Biological Chemistry. 2015; Vol.290:30797-30805. ©the American Society for Biochemistry and Molecular Biology.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/60457
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 小原 圭介

 

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