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Protein oxidation mediated by heme-induced active site conversion specific for heme-regulated transcription factor, iron response regulator

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この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/60742

タイトル: Protein oxidation mediated by heme-induced active site conversion specific for heme-regulated transcription factor, iron response regulator
著者: Kitatsuji, Chihiro 著作を一覧する
Izumi, Kozue 著作を一覧する
Nambu, Shusuke 著作を一覧する
Kurogochi, Masaki 著作を一覧する
Uchida, Takeshi 著作を一覧する
Nishimura, Shin-Ichiro 著作を一覧する
Iwai, Kazuhiro 著作を一覧する
O'Brian, Mark R. 著作を一覧する
Ikeda-Saito, Masao 著作を一覧する
Ishimori, Koichiro 著作を一覧する
発行日: 2016年 1月 5日
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 6
開始ページ: 18703
出版社 DOI: 10.1038/srep18703
抄録: The Bradyrhizobium japonicum transcriptional regulator Irr (iron response regulator) is a key regulator of the iron homeostasis, which is degraded in response to heme binding via a mechanism that involves oxidative modification of the protein. Here, we show that heme-bound Irr activates O-2 to form highly reactive oxygen species (ROS) with the "active site conversion" from heme iron to non-heme iron to degrade itself. In the presence of heme and reductant, the ROS scavenging experiments show that Irr generates H2O2 from O-2 as found for other hemoproteins, but H2O2 is less effective in oxidizing the peptide, and further activation of H2O2 is suggested. Interestingly, we find a time-dependent decrease of the intensity of the Soret band and appearance of the characteristic EPR signal at g = 4.3 during the oxidation, showing the heme degradation and the successive formation of a non-heme iron site. Together with the mutational studies, we here propose a novel "two-step self-oxidative modification" mechanism, during which O-2 is activated to form H2O2 at the heme regulatory motif (HRM) site and the generated H2O2 is further converted into more reactive species such as OH at the non-heme iron site in the His-cluster region formed by the active site conversion.
資料タイプ: article
URI: http://hdl.handle.net/2115/60742
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 石森 浩一郎

 

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