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Post-translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet

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Title: Post-translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet
Authors: Kitazaki, Kazuyoshi Browse this author
Arakawa, Takumi Browse this author
Matsunaga, Muneyuki Browse this author
Yui-Kurino, Rika Browse this author
Matsuhira, Hiroaki Browse this author
Mikami, Tetsuo Browse this author →KAKEN DB
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: cytoplasmic male sterility
fertility restoration
plant mitochondria
protein-protein interaction
Beta vulgaris ssp. vulgaris
Blue Native polyacrylamide gel electrophoresis
Issue Date: 4-Jul-2015
Publisher: Wiley-blackwell
Journal Title: The Plant Journal
Volume: 83
Issue: 2
Start Page: 290
End Page: 299
Publisher DOI: 10.1111/tpj.12888
PMID: 26031622
Abstract: Genetic conflict between cytoplasmically inherited elements and nuclear genes due to their different transmission patterns can be seen in cytoplasmic male sterility (CMS), the mitochondrion-encoded inability to shed functional pollen. CMS is associated with a mitochondrial ORF absent from non sterility-inducing mitochondria (S-orf). Nuclear genes that suppress CMS are called restorer-of-fertility (Rf) genes. Post-transcriptional and translational repression of S-orf mediates the molecular action of Rf that encodes a class of RNA-binding proteins having pentatricopeptide repeat (PPR) motifs. Besides the PPR-type of Rfs, there are also non-PPR Rfs, but the molecular interactions between non-PPR Rf and S-orf have not been described. In this study, we investigated the interaction of sugar beet bvORF20, a non-PPR Rf, with preSatp6, the sugar beet S-orf. Anthers expressing bvORF20 contained a protein that interacted with preSATP6 protein. Analysis of anthers and transgenic calli expressing a FLAG-tagged bvORF20 suggested binding of preSATP6 to bvORF20. To see the effect of bvORF20 on preSATP6, which exists as a 250-kDa protein-complex in CMS plants, signal bands of preSATP6 in bvORF20-expressing and non-expressing anthers were compared by immunoblotting combined with Blue Native polyacrylamide gel electrophoresis. The signal intensity of the 250-kDa band decreased significantly and 200- and 150-kDa bands appeared in bvORF20-expressing anthers. Transgenic callus expressing bvORF20 also generated the 200- and 150-kDa bands. The 200-kDa complex likely includes both preSATP6 and bvORF20. Post-translational interaction between preSATP6 and bvORF20 appears to alter the higher order structure of preSATP6 that may lead to fertility restoration in sugar beet.
Rights: This is the peer reviewed version of the following article: "Post-translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet", The Plant Journal, 83(2)pp290-299, which has been published in final form at 10.1111/tpj.12888. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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