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Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses

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Title: Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses
Authors: Nakajima, Shunsuke Browse this author
Satoh, Yasuharu Browse this author →KAKEN DB
Yanashima, Kentaro Browse this author
Matsui, Tomomi Browse this author
Dairi, Tohru Browse this author →KAKEN DB
Keywords: Ergothioneine
Streptomyces coelicolor
Biological function
Oxidative stress
Issue Date: Sep-2015
Publisher: Society for Bioscience and Bioengineering
Journal Title: Journal of bioscience and bioengineering
Volume: 120
Issue: 3
Start Page: 294
End Page: 298
Publisher DOI: 10.1016/j.jbiosc.2015.01.013
PMID: 25683449
Abstract: Thiol compounds with low-molecular weight, such as glutathione, mycothiol (MSH), bacillithiol, and ergothioneine (ERG), are known to protect microorganisms from oxidative stresses. Mycobacteria and actinobacteria utilize both MSH and ERG. The biological functions of MSH in mycobacteria have been extensively studied by genetic and biochemical studies, which have suggested it has critical roles for detoxification in cells. In contrast, the biological functions of ERG remain ambiguous because its biosynthetic genes were only recently identified in Mycobacterium avium. In this study, we constructed mutants of Streptomyces coelicolor A3(2), in which either the MSH or ERG biosynthetic gene was disrupted, and examined their phenotypes. A mshC (SC01663)-disruptant completely lost MSH productivity. In contrast, a disruptant of the egtA gene (SC00910) encoding gamma-glutamyl-cysteine synthetase unexpectedly retained reduced productivity of ERG, probably because of the use of L-cysteine instead of gamma-glutamyl-cysteine. Both disruptants showed delayed growth at the late logarithmic phase and were more susceptible to hydrogen peroxide and cumene hydroperoxide than the parental strain. Interestingly, the ERG-disruptant, which still kept reduced ERG productivity, was more susceptible. Furthermore, the ERG-disruptant accumulated 5-fold more MSH than the parental strain. In contrast, the amount of ERG was almost the same between the MSH-disruptant and the parental strain. Taken together, our results suggest that ERG is more important than MSH in S. coelicolor A3(2).
Rights: © 2015 The Society for Biotechnology, Japan
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大利 徹

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