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Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : age-dependent desensitization and restoration under inflammatory conditions in mice

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タイトル: Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : age-dependent desensitization and restoration under inflammatory conditions in mice
著者: Yajima, Masako 著作を一覧する
Kimura, Shunsuke 著作を一覧する
Karaki, Shinichiro 著作を一覧する
Nio-Kobayashi, Junko 著作を一覧する
Tsuruta, Takeshi 著作を一覧する
Kuwahara, Atsukazu 著作を一覧する
Yajima, Takaji 著作を一覧する
Iwanaga, Toshihiko 著作を一覧する
キーワード: Acetylcholinesterase
age
ileal contraction
inflammatory condition
non-neuronal acetylcholine
short-chain fatty acids
発行日: 2016年 4月
出版者: Wiley
誌名: Physiological reports
巻: 4
号: 7
開始ページ: e12759
出版社 DOI: 10.14814/phy2.12759
抄録: Intestinal epithelial cells sense short-chain fatty acids (SCFAs) to secrete non-neuronal acetylcholine (ACh). However, the roles of luminal SCFAs and epithelial ACh under normal and pathological conditions remain unknown. We examined ileal contractile responses to SCFAs at different ages and their mucosal cholinergic alterations under inflammatory conditions. Ileal contractile responses to SCFAs in 1-day-old pups to 7-week-old mice were compared using an isotonic transducer, and responses to an intraperitoneal injection of lipopolysaccharide (LPS) were analyzed in 7-week-old mice. The mRNA expression levels of a SCFA activate free fatty acid receptor, acetylcholinesterase (AChE), choline acetyltransferase (Chat), and choline transporter-like protein 4 (CTL4) were measured using real-time quantitative RT-PCR. AChE was analyzed by histochemical and optical enzymatic assays. Atropine-sensitive ileal contractile responses to SCFAs occurred in all 1-day-old pups, but were frequently desensitized after the weaning period. These contractile responses were not inhibited by tetrodotoxin and did not appear when the mucosal layer had been scraped off. Contractile desensitization in 7-week-old mice was abolished in the presence of the AChE inhibitor, eserine, which was consistent with increased AChE activity after weaning. Ileal contractions to SCFAs in adult mice were restored by LPS, which significantly increased the epithelial mRNA expression of Chat and CTL4. Atropine-sensitive ileal contractile responses to SCFAs constitutively occur in the newborn period, and are desensitized during developmental stages following the up-regulated expression of AChE in the villous mucosa, but are restored under inflammatory conditions possibly via the release of epithelial ACh.
資料タイプ: article
URI: http://hdl.handle.net/2115/62800
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 矢島 昌子

 

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