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Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : age-dependent desensitization and restoration under inflammatory conditions in mice

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/62800

Title: Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : age-dependent desensitization and restoration under inflammatory conditions in mice
Authors: Yajima, Masako Browse this author
Kimura, Shunsuke Browse this author →KAKEN DB
Karaki, Shinichiro Browse this author
Nio-Kobayashi, Junko Browse this author →KAKEN DB
Tsuruta, Takeshi Browse this author
Kuwahara, Atsukazu Browse this author
Yajima, Takaji Browse this author
Iwanaga, Toshihiko Browse this author →KAKEN DB
Keywords: Acetylcholinesterase
age
ileal contraction
inflammatory condition
non-neuronal acetylcholine
short-chain fatty acids
Issue Date: Apr-2016
Publisher: Wiley
Journal Title: Physiological reports
Volume: 4
Issue: 7
Start Page: e12759
Publisher DOI: 10.14814/phy2.12759
Abstract: Intestinal epithelial cells sense short-chain fatty acids (SCFAs) to secrete non-neuronal acetylcholine (ACh). However, the roles of luminal SCFAs and epithelial ACh under normal and pathological conditions remain unknown. We examined ileal contractile responses to SCFAs at different ages and their mucosal cholinergic alterations under inflammatory conditions. Ileal contractile responses to SCFAs in 1-day-old pups to 7-week-old mice were compared using an isotonic transducer, and responses to an intraperitoneal injection of lipopolysaccharide (LPS) were analyzed in 7-week-old mice. The mRNA expression levels of a SCFA activate free fatty acid receptor, acetylcholinesterase (AChE), choline acetyltransferase (Chat), and choline transporter-like protein 4 (CTL4) were measured using real-time quantitative RT-PCR. AChE was analyzed by histochemical and optical enzymatic assays. Atropine-sensitive ileal contractile responses to SCFAs occurred in all 1-day-old pups, but were frequently desensitized after the weaning period. These contractile responses were not inhibited by tetrodotoxin and did not appear when the mucosal layer had been scraped off. Contractile desensitization in 7-week-old mice was abolished in the presence of the AChE inhibitor, eserine, which was consistent with increased AChE activity after weaning. Ileal contractions to SCFAs in adult mice were restored by LPS, which significantly increased the epithelial mRNA expression of Chat and CTL4. Atropine-sensitive ileal contractile responses to SCFAs constitutively occur in the newborn period, and are desensitized during developmental stages following the up-regulated expression of AChE in the villous mucosa, but are restored under inflammatory conditions possibly via the release of epithelial ACh.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/62800
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 矢島 昌子

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