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Relationship Between the Physicochemical Properties of Lipid Nanoparticles and the Quality of siRNA Delivery to Liver Cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/62957

Title: Relationship Between the Physicochemical Properties of Lipid Nanoparticles and the Quality of siRNA Delivery to Liver Cells
Authors: Sato, Yusuke Browse this author →KAKEN DB
Hatakeyama, Hiroto Browse this author →KAKEN DB
Hyodo, Mamoru Browse this author →KAKEN DB
Harashima, Hideyoshi Browse this author →KAKEN DB
Issue Date: Apr-2016
Publisher: Nature Publishing Group
Journal Title: Molecular therapy
Volume: 24
Issue: 4
Start Page: 788
End Page: 795
Publisher DOI: 10.1038/mt.2015.222
Abstract: While a variety of short interfering RNA (siRNA) delivery compounds have been developed, a deep understanding of the key parameters that determine the quality of siRNA delivery are not known with certainty. Therefore, an understanding of the factors required for the efficient, selective, and safe delivery of siRNA is a great challenge for successful siRNA delivery. Herein, we report on the development of two pH-sensitive cationic lipids and their use in examining the impact of the acid dissociation constant (pK(a)) value, lipase sensitivity and the size of lipid nanoparticles on the biodistribution, and efficiency and cell specificity of gene silencing in the liver. An increase in the pK(a) value resulted in a significant change in the intrahepatic localization of siRNA and gene-silencing efficiency in hepatocytes and liver sinusoidal endothelial cells (LSECs). The sensitivity of the pH-sensitive cationic lipid to lipases was a major factor in achieving hepatocyte-specific gene silencing. Increasing the particle size can improve the LSEC specificity of gene silencing. As a consequence, we succeeded in developing both a highly efficient, hepatocyte-specific formulation, and the most efficacious LSEC-targeted formulation reported to date. These findings will facilitate the development of more sophisticated siRNA delivery systems.
Type: article (author version)
URI: http://hdl.handle.net/2115/62957
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 悠介

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