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Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells

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タイトル: Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells
著者: Kamada, Rui 著作を一覧する
Tano, Fumi 著作を一覧する
Kudoh, Fuki 著作を一覧する
Kimura, Nozomi 著作を一覧する
Chuman, Yoshiro 著作を一覧する
Osawa, Ayumi 著作を一覧する
Namba, Kosuke 著作を一覧する
Tanino, Keiji 著作を一覧する
Sakaguchi, Kazuyasu 著作を一覧する
発行日: 2016年 8月 4日
出版者: Public Library of Science
誌名: PLOS ONE
巻: 11
号: 8
開始ページ: e0160625
出版社 DOI: 10.1371/journal.pone.0160625
抄録: Nuclear and cytoplasmic morphological changes provide important information about cell differentiation processes, cell functions, and signal responses. There is a strong desire to develop a rapid and simple method for visualizing cytoplasmic and nuclear morphology. Here, we developed a novel and rapid method for probing cellular morphological changes of live cell differentiation process by a fluorescent probe, TAP-4PH, a 1,3a,6a-triazapentalene derivative. TAP-4PH showed high fluorescence in cytoplasmic area, and visualized cytoplasmic and nuclear morphological changes of live cells during differentiation. We demonstrated that TAP-4PH visualized dendritic axon and spine formation in neuronal differentiation, and nuclear structural changes during neutrophilic differentiation. We also showed that the utility of TAP-4PH for visualization of cytoplasmic and nuclear morphologies of various type of live cells. Our visualizing method has no toxicity and no influence on the cellular differentiation and function. The cell morphology can be rapidly observed after addition of TAP-4PH and can continue to be observed in the presence of TAP-4PH in cell culture medium. Moreover, TAP-4PH can be easily removed after observation by washing for subsequent biological assay. Taken together, these results demonstrate that our visualization method is a powerful tool to probe differentiation processes before subsequent biological assay in live cells.
資料タイプ: article
URI: http://hdl.handle.net/2115/63136
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 坂口 和靖

 

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