HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
工学研究科  >
雑誌発表論文等  >

An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas


Science and Technology of Advanced Materials_16_17_618_Yamamichi.pdf1.09 MBPDF見る/開く

タイトル: An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
著者: Yamamichi, Akane 著作を一覧する
Kasama, Toshihiro 著作を一覧する
Ohka, Fumiharu 著作を一覧する
Suzuki, Hiromichi 著作を一覧する
Kato, Akira 著作を一覧する
Motomura, Kazuya 著作を一覧する
Hirano, Masaki 著作を一覧する
Ranjit, Melissa 著作を一覧する
Chalise, Lushun 著作を一覧する
Kurimoto, Michihiro 著作を一覧する
Kondo, Goro 著作を一覧する
Aoki, Kosuke 著作を一覧する
Kaji, Noritada 著作を一覧する
Tokeshi, Manabu 著作を一覧する
Matsubara, Toshio 著作を一覧する
Senga, Takeshi 著作を一覧する
Kaneko, Mika K. 著作を一覧する
Suzuki, Hidenori 著作を一覧する
Hara, Masahito 著作を一覧する
Wakabayashi, Toshihiko 著作を一覧する
Baba, Yoshinobu 著作を一覧する
Kato, Yukinari 著作を一覧する
Natsume, Atsushi 著作を一覧する
キーワード: Glioma
isocitrate dehydrogenase 1 mutation
immuno-wall microdevice
rapid diagnosis
precision medicine
発行日: 2016年10月
出版者: Taylor & Francis
誌名: Science and Technology of Advanced Materials
巻: 17
号: 1
開始ページ: 618
終了ページ: 625
出版社 DOI: 10.1080/14686996.2016.1227222
抄録: World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 渡慶次 学


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学