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An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas

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Title: An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
Authors: Yamamichi, Akane Browse this author
Kasama, Toshihiro Browse this author
Ohka, Fumiharu Browse this author
Suzuki, Hiromichi Browse this author
Kato, Akira Browse this author
Motomura, Kazuya Browse this author
Hirano, Masaki Browse this author
Ranjit, Melissa Browse this author
Chalise, Lushun Browse this author
Kurimoto, Michihiro Browse this author
Kondo, Goro Browse this author
Aoki, Kosuke Browse this author
Kaji, Noritada Browse this author →KAKEN DB
Tokeshi, Manabu Browse this author →KAKEN DB
Matsubara, Toshio Browse this author
Senga, Takeshi Browse this author
Kaneko, Mika K. Browse this author
Suzuki, Hidenori Browse this author
Hara, Masahito Browse this author
Wakabayashi, Toshihiko Browse this author
Baba, Yoshinobu Browse this author →KAKEN DB
Kato, Yukinari Browse this author
Natsume, Atsushi Browse this author
Keywords: Glioma
isocitrate dehydrogenase 1 mutation
immuno-wall microdevice
rapid diagnosis
precision medicine
Issue Date: Oct-2016
Publisher: Taylor & Francis
Journal Title: Science and Technology of Advanced Materials
Volume: 17
Issue: 1
Start Page: 618
End Page: 625
Publisher DOI: 10.1080/14686996.2016.1227222
Abstract: World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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