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Label-free detection of real-time DNA amplification using a nanofluidic diffraction grating

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/63210

Title: Label-free detection of real-time DNA amplification using a nanofluidic diffraction grating
Authors: Yasui, Takao Browse this author
Ogawa, Kensuke Browse this author
Kaji, Noritada Browse this author →KAKEN DB
Nilsson, Mats Browse this author
Ajiri, Taiga Browse this author
Tokeshi, Manabu Browse this author →KAKEN DB
Horiike, Yasuhiro Browse this author
Baba, Yoshinobu Browse this author →KAKEN DB
Issue Date: 17-Aug-2016
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 6
Start Page: 31642
Publisher DOI: 10.1038/srep31642
Abstract: Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological research. Here we report a label-free detection of real-time DNA amplification using a nanofluidic diffraction grating. Our detection system observed intensity changes during DNA amplification of diffracted light derived from the passage of a laser beam through nanochannels embedded in a microchannel. Numerical simulations revealed that the diffracted light intensity change in the nanofluidic diffraction grating was attributed to the change of refractive index. We showed the first case reported to date for label-free detection of real-time DNA amplification, such as specific DNA sequences from tubercle bacilli (TB) and human papillomavirus (HPV). Since our developed system allows quantification of the initial concentration of amplified DNA molecules ranging from 1 fM to 1 pM, we expect that it will offer a new strategy for developing fundamental techniques of medical applications.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/63210
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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