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A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space

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Title: A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space
Authors: Maeki, Masatoshi Browse this author
Yamazaki, Shohei Browse this author
Pawate, Ashtamurthy S. Browse this author
Ishida, Akihiko Browse this author →KAKEN DB
Tani, Hirofumi Browse this author →KAKEN DB
Yamashita, Kenichi Browse this author
Sugishima, Masakazu Browse this author
Watanabe, Keiichi Browse this author
Tokeshi, Manabu Browse this author →KAKEN DB
Kenis, Paul J. A. Browse this author
Miyazaki, Masaya Browse this author
Issue Date: 28-Oct-2016
Publisher: Royal Society of Chemistry
Journal Title: CrystEngComm
Volume: 18
Issue: 40
Start Page: 7722
End Page: 7727
Publisher DOI: 10.1039/C6CE01671E
Abstract: Protein crystallization and subsequent X-ray diffraction analysis of the three-dimensional structure are necessary for elucidation of the biological functions of proteins and effective rational drug design. Therefore, controlling protein crystallization is important to obtain high resolution X-ray diffraction data. Here, a simple microfluidic method using chips with 10 and 50 μm high crystallization chambers to selectively form suitable single protein crystals for X-ray analysis is demonstrated. As proof of concept, three different types of proteins: lysozyme, glucokinase from Pseudoalteromonas sp. AS-131 (PsGK), and NADPH-cytochrome P450 oxidoreductase–heme oxygenase complex were crystallized. We demonstrate that the crystal growth orientation depends on the height of the crystallization chamber regardless of the protein type. Our results suggest that the confined micro space induces the protein molecules to adhere to a specific crystal face and affects the growth kinetics of each crystal face. The present microfluidic-based protein crystallization method can reform a suitable single protein crystal for X-ray analysis from aggregates of needle-shaped protein crystals.
Rights: https://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/63212
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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