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Molecular mechanism of male differentiation is conserved in the SRY-absent mammal, Tokudaia osimensis

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タイトル: Molecular mechanism of male differentiation is conserved in the SRY-absent mammal, Tokudaia osimensis
著者: Otake, Tomofumi 著作を一覧する
Kuroiwa, Asato 著作を一覧する
発行日: 2016年 9月10日
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 6
開始ページ: 32874
出版社 DOI: 10.1038/srep32874
抄録: The sex-determining gene SRY induces SOX9 expression in the testes of eutherian mammals via two pathways. SRY binds to testis-specific enhancer of Sox9 (TESCO) with SF1 to activate SOX9 transcription. SRY also up-regulates ER71 expression, and ER71 activates Sox9 transcription. After the initiation of testis differentiation, SOX9 enhances Amh expression by binding to its promoter with SF1. SOX8, SOX9 and SOX10, members of the SOXE gene family, also enhance the activities of the Amh promoter and TESCO. In this study, we investigated the regulation of these sexual differentiation genes in Tokudaia osimensis, which lacks a Y chromosome and the SRY gene. The activity of the AMH promoter was stimulated by SOXE genes and SF1. Mutant AMH promoters, with mutations in its SOX and SF1 binding sites, did not show significant activity by SOX9 and SF1. These results indicate that AMH expression was regulated by the binding of SOX9 and SF1. By contrast, SOXE genes could not enhance TESCO activity. These results indicate that TESCO enhancer activity was lost in this species. Furthermore, the activity of the SOX9 promoter was enhanced by ER71, indicating that ER71 may play an important role in the testis-specific expression of SOX9.
資料タイプ: article
URI: http://hdl.handle.net/2115/63213
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 黒岩 麻里

 

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