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Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines
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| Title: | Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines |
| Authors: | Muramatsu, Daisuke Browse this author | | Kawata, Koji Browse this author | | Aoki, Shiho Browse this author | | Uchiyama, Hirofumi Browse this author | | Okabe, Mitsuyasu Browse this author | | Miyazaki, Tadaaki Browse this author →KAKEN DB | | Kida, Hiroshi Browse this author →KAKEN DB | | Iwai, Atsushi Browse this author |
| Keywords: | Extracellular signalling molecules | | Interferons |
| Issue Date: | 24-Apr-2014 |
| Publisher: | Nature |
| Journal Title: | Scientific Reports |
| Volume: | 4 |
| Start Page: | 4777 |
| Publisher DOI: | 10.1038/srep04777 |
| Abstract: | A β-(1,3),(1,6)-D-glucan produced by A. pullulans (AP-PG) is known to be an immune stimulating agent. In this study, we demonstrate that the stimulation with AP-PG effectively induces the interferon (IFN) stimulated genes (ISGs) in macrophage-like cell lines. The ISGs, Mx1, ISG15, and viperin mRNAs were significantly increased in RAW264.7 cells after stimulation with AP-PG. The stimulation with AP-PG transiently induced IFN-β mRNA. However, the expression of viperin mRNA was also increased after stimulation with AP-PG even when new protein synthesis was completely blocked by treatment with cycloheximide. Further, in IFN-α receptor knockdown RAW264.7 cells, AP-PG stimulation more effectively induced viperin mRNA compared with that of IFN-α stimulation. The phosphorylation of Ser 727 in STAT1 involved in the enhancement of STAT1 activation was immediately increased after stimulation with AP-PG. In addition, viperin mRNA expression induced after stimulation with IFN-α was significantly increased by combined stimulation with AP-PG. These results suggest that stimulation with AP-PG effectively induces the ISGs through the induction of IFN and the enhancement of STAT1-mediated transcriptional activation. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63432 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岩井 淳
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